Antigen-specific T cell immune response detected by skewed T cell receptor usage in normal placenta and complete molar pregnancy

Zoltan Nagymanyoki, Marcus O. Butler, Michael J. Callahan, Mana M. Parast, V. Fülöp, Samuel C. Mok, Ross S. Berkowitz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: To investigate the antigenicity of normal placenta and complete molar trophoblastic tissue. STUDY DESIGN: T cell receptor (TCR) variable beta chain (VBC) gene expression was analyzed utilizing fresh frozen tissues. Real-time polymerase chain reactions (RT-PCR) were performed on cDNA samples from 10 normal buffy coats (BC), 7 normal placentas (NP) and 14 complete molar pregnancies (CM) using a TCR beta chain and 25 variable TCR beta chain primers. Relative expressions were calculated for each individual gene. RESULTS: Signficant changes were noted in most of the gene expressions in NP and CM as compared to the buffy coat samples. The relative expression of most venes was significantly decreased in NP and CM, but VBC gene number 4 was increased in both NP and CM; however, a significant difference was noted only between BC and CM (p = 0.023). Comparing NP to CM, 5 other VBC gene expressions were decreased significantly in the CM tissues (p <0.05). CONCLUSION: In normal placenta and CM pregnancies. T cells appear to express certain TCR VBC genes in a different manner than in BC. These genes and the VBC gene profile difference found between NP and CM may play an important role in the immunobiology of CM pregnancy.

Original languageEnglish
Pages (from-to)528-534
Number of pages7
JournalJournal of Reproductive Medicine for the Obstetrician and Gynecologist
Volume53
Issue number7
Publication statusPublished - Jul 2008

Fingerprint

Hydatidiform Mole
T-Cell Antigen Receptor
Placenta
T-Lymphocytes
Antigens
T-Cell Receptor beta Genes
Antigen Receptors, T-Cell, alpha-beta
Gene Expression
Genes
Pregnancy
Real-Time Polymerase Chain Reaction
Complementary DNA

Keywords

  • Complete mole
  • Gestational trophoblastic disease
  • Pregnancy
  • T cell receptor
  • Variable beta chain

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

Cite this

Antigen-specific T cell immune response detected by skewed T cell receptor usage in normal placenta and complete molar pregnancy. / Nagymanyoki, Zoltan; Butler, Marcus O.; Callahan, Michael J.; Parast, Mana M.; Fülöp, V.; Mok, Samuel C.; Berkowitz, Ross S.

In: Journal of Reproductive Medicine for the Obstetrician and Gynecologist, Vol. 53, No. 7, 07.2008, p. 528-534.

Research output: Contribution to journalArticle

Nagymanyoki, Zoltan ; Butler, Marcus O. ; Callahan, Michael J. ; Parast, Mana M. ; Fülöp, V. ; Mok, Samuel C. ; Berkowitz, Ross S. / Antigen-specific T cell immune response detected by skewed T cell receptor usage in normal placenta and complete molar pregnancy. In: Journal of Reproductive Medicine for the Obstetrician and Gynecologist. 2008 ; Vol. 53, No. 7. pp. 528-534.
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