Antigen-specific immunity does not mediate acute regression of UVB-induced p53-mutant clones

E. Remenyik, N. Wikonkál, Wengeng Zhang, Vipin Paliwal, Douglas E. Brash

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Chronic irradiation of human or murine epidermis with ultraviolet B (UVB) induces clones of p53-mutant keratinocytes. Clones precede and parallel the induction of carcinomas, suggesting that they are an early stage of UVB carcinogenesis. In the absence of UVB, these clones rapidly regress. For UVB-induced murine skin tumors and papillomas, regression is known to involve antigen-specific immunity. To determine whether antigen-specific immunity influences the creation, expansion, or regression of p53-mutant clones, we studied Rag1 knockout mice deficient in the recombination activating gene 1 required for development of B, αβT, γδT, and natural killer T cells. Since tissue homeostasis could affect proliferation or persistence of clones, we also examined the effect of Rag1 on UVB-induced hyperplasia and apoptosis. Mice were irradiated with UVB daily for 7-11 weeks to create p53-mutant clones, and then retained in the absence of UV. After UV ended, epidermal thickness decreased and p53-mutant clones observed in the epidermal sheets regressed, with no significant differences between Rag1 -/- and wild type. During the initial chronic UVB irradiation, increasing irradiation time increased both the number and size of p53-mutant clones, with no significant difference between genotypes. We conclude that antigen-specific immunity is not involved in the initiation, expansion, or acute regression of p53-mutant clones.

Original languageEnglish
Pages (from-to)6369-6376
Number of pages8
JournalOncogene
Volume22
Issue number41
DOIs
Publication statusPublished - Sep 25 2003

Fingerprint

Immunity
Clone Cells
Antigens
RAG-1 Genes
Natural Killer T-Cells
Papilloma
Keratinocytes
Epidermis
Knockout Mice
Hyperplasia
Carcinogenesis
Homeostasis
Genotype
Apoptosis
Carcinoma
Skin
Neoplasms

Keywords

  • Apoptosis
  • Epidermal thickness
  • Immune surveillance
  • Knockout
  • Rag1
  • Sunburn cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Antigen-specific immunity does not mediate acute regression of UVB-induced p53-mutant clones. / Remenyik, E.; Wikonkál, N.; Zhang, Wengeng; Paliwal, Vipin; Brash, Douglas E.

In: Oncogene, Vol. 22, No. 41, 25.09.2003, p. 6369-6376.

Research output: Contribution to journalArticle

Remenyik, E. ; Wikonkál, N. ; Zhang, Wengeng ; Paliwal, Vipin ; Brash, Douglas E. / Antigen-specific immunity does not mediate acute regression of UVB-induced p53-mutant clones. In: Oncogene. 2003 ; Vol. 22, No. 41. pp. 6369-6376.
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