Antigen receptor-mediated signaling pathways in transitional immature B cells

Dorottya Kövesdi, K. Pászty, A. Enyedi, Endre Kiss, J. Matkó, Katalin Ludányi, E. Rajnavolgyi, G. Sármay

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Engagement of antigen receptors on immature B cells induces apoptosis, while at the mature stage, it stimulates cell activation and proliferation. The difference in B cell receptor (BCR)-mediated signaling pathways regulating death or survival of B cells is not fully understood. We aimed to characterize the pathway leading to BCR-driven apoptosis. Transitional immature B cells were obtained from the spleen of sublethally irradiated and auto-reconstituted mice. We have detected a short-lived BCR-driven activation of mitogen-activated protein kinases (ERK1/2 and p38 MAPK) and Akt/PKB in transitional immature B cells that correlated with the lack of c-Fos expression, reduced phosphorylation of Akt substrates and a susceptibility for apoptosis. Simultaneous signaling through BCR and CD40 protected immature B cells from apoptosis, however, without inducing Bcl-2 expression. The BCR-induced apoptosis of immature B cells is a result of the collapse of mitochondrial membrane potential and the subsequent activation of caspase-3.

Original languageEnglish
Pages (from-to)881-889
Number of pages9
JournalCellular Signalling
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Antigen Receptors
B-Lymphoid Precursor Cells
B-Lymphocytes
Apoptosis
p38 Mitogen-Activated Protein Kinases
Mitochondrial Membrane Potential
Mitogen-Activated Protein Kinase 1
Caspase 3
Spleen
Phosphorylation
Cell Proliferation

Keywords

  • Apoptosis
  • B lymphocytes
  • Development
  • Signal transduction
  • Transitional immature cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Antigen receptor-mediated signaling pathways in transitional immature B cells. / Kövesdi, Dorottya; Pászty, K.; Enyedi, A.; Kiss, Endre; Matkó, J.; Ludányi, Katalin; Rajnavolgyi, E.; Sármay, G.

In: Cellular Signalling, Vol. 16, No. 8, 08.2004, p. 881-889.

Research output: Contribution to journalArticle

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