Antigen-antibody complexes bound to B-lymphocyte Fcγ receptors regulate B-lymphocyte differentiation

F. Uher, Marinus C. Lamers, Howard B. Dickler

Research output: Contribution to journalArticle

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Abstract

We studied the effect of soluble antigen-antibody complexes on the responses of polyclonally activated murine B lymphocytes. For this, normal B lymphocytes were stimulated with rabbit F(ab′)2 anti-μ and lymphokines. IgG complexes, particularly in antigen excess, inhibited the plaque-forming cell response (55-70%), while proliferation was unaffected. Maximal inhibition was obtained with small amounts (0.2-1.0 μg/ml) of complexes. Neither antigen or antibody alone was inhibitory. Inhibition was mediated via binding of the IgG complexes to Fcγ receptors of B lymphocytes: (1) neither T lymphocytes or adherent accessory cells were required; (2) IgM complexes did not inhibit; and (3) inhibition was not seen when monoclonal anti-Fcγ receptor antibodies prevented binding of the IgG complexes to these receptors. Kinetic experiments showed that B lymphocytes are susceptible to this inhibitory signal for only a short time after stimulation. We conclude that IgG complexes bound to the Fcγ receptors of B lymphocytes regulate B-lymphocyte differentiation.

Original languageEnglish
Pages (from-to)368-379
Number of pages12
JournalCellular Immunology
Volume95
Issue number2
DOIs
Publication statusPublished - Oct 15 1985

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Fc Receptors
Antigen-Antibody Complex
B-Lymphocytes
Immunoglobulin G
Antigens
Antibodies
Lymphokines
Immunoglobulin M
Rabbits
T-Lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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Antigen-antibody complexes bound to B-lymphocyte Fcγ receptors regulate B-lymphocyte differentiation. / Uher, F.; Lamers, Marinus C.; Dickler, Howard B.

In: Cellular Immunology, Vol. 95, No. 2, 15.10.1985, p. 368-379.

Research output: Contribution to journalArticle

Uher, F. ; Lamers, Marinus C. ; Dickler, Howard B. / Antigen-antibody complexes bound to B-lymphocyte Fcγ receptors regulate B-lymphocyte differentiation. In: Cellular Immunology. 1985 ; Vol. 95, No. 2. pp. 368-379.
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