Antifungal peptides homologous to the Penicillium chrysogenum antifungal protein (PAF) are widespread among Fusaria

László Galgóczy, Máté Virágh, Laura Kovács, Beáta Tóth, Tamás Papp, Csaba Vágvölgyi

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14 Citations (Scopus)

Abstract

Putative antifungal peptide encoding genes containing Penicillium chrysogenum antifungal protein (PAF) characteristic amino acid motifs were identified in 15 Fusarium isolates, representing 10 species. Based on the predicted sequences of mature peptides, discrepancy in one, two or three amino acids was observed between them. Phylogenetic investigations revealed that they show high amino acid sequence similarity to PAF and they belong to the group of fungal derived antifungal peptides with PAF-cluster. Ten from the 15 partially purified <10 kDa peptide fraction of Fusarium ferment broths showed antifungal activity. The presence of approximately 6.3 kDa molecular weight peptides was detected in all of the antifungally active ferment broths, and this peptide was isolated and purified from Fusarium polyphilaidicum. The minimal inhibitiory concentrations of F. polyphilaidicum antifungal protein (FPAP) were determined against different filamentous fungi, yeasts and bacteria. Filamentous fungal species were the most susceptible to FPAF, but some yeasts were also slightly sensitive.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalPeptides
Volume39
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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Keywords

  • Antimicrobial susceptibility
  • Fusarium polyphialidicum antifungal protein (FPAP)
  • Fusarium spp.
  • Penicillium chrysogenum antifungal protein (PAF)
  • Peptide isolation
  • Phylogenetic relationship

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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