Antibody repertoire development in fetal and neonatal piglets. VIII. Colonization is required for newborn piglets to make serum antibodies to T-dependent and type 2 T-independent antigens

John E. Butler, Patrick Weber, Marek Sinkora, Diane Baker, Amanda Schoenherr, B. Mayer, David Francis

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Cesarean-derived piglets were reared for 5 wk under germfree conditions or monoassociated with a benign Escherichia coli (G58-1) or a enterohemorrhagic strain (933D) derived from O157:H7, and immunized i.p. with the T-dependent (TD) Ags fluorescein-labeled (FL) keyhole limpet hemocyanin or trinitrophenylated (TNP) keyhole limpet hemocyanin and the type 2 T-independent Ags TNP-Ficoll or FL-Ficoll. Only colonized piglets showed an increase in serum IgG, IgA, and IgM and had serum Abs to FL, TNP, and colonizing bacteria. While serum Abs to FL or TNP appeared following colonization alone, secondary responses were restricted to piglets immunized using TD carriers. While animals colonized with 933D had significantly higher total serum IgG and IgM levels and specific IgG Abs than those colonized with G58-1, no differences were seen in serum IgA levels, B cell diversification in the ileal Peyer's patches, and specific activity (ELISA activity per micrograms of Ig) of pre-boost serum IgG and IgM anti-TNP and anti-FL Abs. Serum IgA Abs to TNP, FL, or bacteria were not detected. Ag-driven responses, as measured by an increase in specific Ab activity, were only observed in secondary responses to TD Ags and to colonizing, pathogenic E. coli. We propose that germline-encoded, isotype-switched B cells in newborn piglets differentiate to Ab-secreting cells 1) after stimulation by bacteria-activated APCs or 2) through direct stimulation by bacterial products. We further propose that Ag-driven systemic responses require both bacterial colonization and TD Ags translocated to the peritoneum.

Original languageEnglish
Pages (from-to)6822-6830
Number of pages9
JournalJournal of Immunology
Volume169
Issue number12
Publication statusPublished - Dec 15 2002

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T Independent Antigens
Fetal Development
Fluorescein
Antibodies
Serum
Immunoglobulin A
Ficoll
Immunoglobulin G
Bacteria
Immunoglobulin M
B-Lymphocytes
Escherichia coli
Peyer's Patches
Peritoneum
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Immunology

Cite this

Antibody repertoire development in fetal and neonatal piglets. VIII. Colonization is required for newborn piglets to make serum antibodies to T-dependent and type 2 T-independent antigens. / Butler, John E.; Weber, Patrick; Sinkora, Marek; Baker, Diane; Schoenherr, Amanda; Mayer, B.; Francis, David.

In: Journal of Immunology, Vol. 169, No. 12, 15.12.2002, p. 6822-6830.

Research output: Contribution to journalArticle

Butler, John E. ; Weber, Patrick ; Sinkora, Marek ; Baker, Diane ; Schoenherr, Amanda ; Mayer, B. ; Francis, David. / Antibody repertoire development in fetal and neonatal piglets. VIII. Colonization is required for newborn piglets to make serum antibodies to T-dependent and type 2 T-independent antigens. In: Journal of Immunology. 2002 ; Vol. 169, No. 12. pp. 6822-6830.
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