Antibiotics delay in vitro human stem cell regrowth

Melinda Turani, Gaspar Banfalvi, Agota Peter, Krisztina Kukoricza, Gabor Kiraly, Laszlo Talas, Bence Tanczos, Balazs Dezso, Gabor Nagy, Adam Kemeny-Beke

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Stem cell line from human limbal area was established to study in vitro cell growth and response to the toxic effects of antibiotics used in ophthalmology in terms of cell migration rates and structure of interphase chromatin. Recovery from cellular damages caused by ophthalmologic antibiotics was mimicked by an in vitro scratch model and followed by time-lapse microscopy, scanning electronmicroscopy and chromatin image analysis. Experiments revealed that broad spectrum antibiotics, chloramphenicol (0.5-1.0. mg/ml) and rifampicin (0.1-0.2. mg/ml), corresponding to concentrations in common clinical practice, slowed down the regeneration process. Results show that nuclei of naturally occurring limbal cells contain the same intermediates of chromatin condensation as seen in mammalian tumor cells and follow the common pathway of chromosome condensation. These intermediates included decondensed veil-like chromatin, fibrillary chromatin, supercoiled ribbon, chromatin bodies, early linear forms and metaphase chromosomes. Upon chloramphenicol and rifampicin treatment characteristic distorsions took place in the intermediates of chromosome condensation. Damaging effects in limbal stem cells in the presence of chloramphenicol or rifampicin indicate that ophthalmologic treatment with antibiotics should be used cautiously.

Original languageEnglish
Pages (from-to)370-379
Number of pages10
JournalToxicology in Vitro
Volume29
Issue number2
DOIs
Publication statusPublished - Mar 1 2015

Keywords

  • Chromatin condensation
  • Fluorescent microscopy
  • Scratch wound mode l
  • Time-lapse imaging
  • Toxicity of antibiotics

ASJC Scopus subject areas

  • Toxicology

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  • Cite this

    Turani, M., Banfalvi, G., Peter, A., Kukoricza, K., Kiraly, G., Talas, L., Tanczos, B., Dezso, B., Nagy, G., & Kemeny-Beke, A. (2015). Antibiotics delay in vitro human stem cell regrowth. Toxicology in Vitro, 29(2), 370-379. https://doi.org/10.1016/j.tiv.2014.10.013