Antibiotic resistance and class 1 integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003

Noémi Nógrády, István Gadó, A. Tóth, J. Pászti

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23 Citations (Scopus)

Abstract

The antibiotic resistance profiles of 5178 Salmonella strains representing 19 non-typhoidal serotypes isolated from human salmonellosis cases in Hungary in 2002 and 2003 were analysed for resistance to 10 antibiotic agents. The most frequent resistances were to nalidixic acid (Nx), streptomycin (S), tetracycline (Tc), ampicillin (Amp) and chloramphenicol (Cm) (ranging from 27% to 13%). Forty-five percent of the Salmonella Typhimurium strains were multiple resistant and belonged mainly to the definitive phage types 104 and U302. A prevalence of 83-94% of strains of serotypes S. Infantis, S. Hadar and S. Virchow was observed with the NxSTc resistance pattern, sometimes complemented with other resistances. Multiple resistance was uncommon in S. Enteritidis; nevertheless, 20% of the strains, most of which belonged to phage type 4, were nalidixic acid resistant. One strain of S. Typhimurium was found to be resistant to ciprofloxacin. Four S. Typhimurium strains were resistant to cefotaxime and produced extended-spectrum β-lactamase. Selected isolates were screened for the presence of class 1 integrons by polymerase chain reaction (PCR). Nucleotide sequencing of the PCR products revealed nine different variable regions. One resistance gene was identified in five variable regions (aadA1, aadA2, aadA23, dfrV and pse-1), and four variable regions carried two resistance gene cassettes (aadB-catB3, dhfrI-aadA, dfrA17-aadA5 and oxa-1-aadA1).

Original languageEnglish
Pages (from-to)126-132
Number of pages7
JournalInternational Journal of Antimicrobial Agents
Volume26
Issue number2
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Integrons
Hungary
Salmonella typhimurium
Microbial Drug Resistance
Salmonella
Nalidixic Acid
Bacteriophages
Polymerase Chain Reaction
Cefotaxime
Salmonella Infections
Streptomycin
Chloramphenicol
Ampicillin
Ciprofloxacin
Tetracycline
Genes
Nucleotides
Anti-Bacterial Agents
Serogroup

Keywords

  • Antibiotic resistance
  • Class 1 integron
  • Multiple resistance
  • Non-typhoidal human salmonellae

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

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title = "Antibiotic resistance and class 1 integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003",
abstract = "The antibiotic resistance profiles of 5178 Salmonella strains representing 19 non-typhoidal serotypes isolated from human salmonellosis cases in Hungary in 2002 and 2003 were analysed for resistance to 10 antibiotic agents. The most frequent resistances were to nalidixic acid (Nx), streptomycin (S), tetracycline (Tc), ampicillin (Amp) and chloramphenicol (Cm) (ranging from 27{\%} to 13{\%}). Forty-five percent of the Salmonella Typhimurium strains were multiple resistant and belonged mainly to the definitive phage types 104 and U302. A prevalence of 83-94{\%} of strains of serotypes S. Infantis, S. Hadar and S. Virchow was observed with the NxSTc resistance pattern, sometimes complemented with other resistances. Multiple resistance was uncommon in S. Enteritidis; nevertheless, 20{\%} of the strains, most of which belonged to phage type 4, were nalidixic acid resistant. One strain of S. Typhimurium was found to be resistant to ciprofloxacin. Four S. Typhimurium strains were resistant to cefotaxime and produced extended-spectrum β-lactamase. Selected isolates were screened for the presence of class 1 integrons by polymerase chain reaction (PCR). Nucleotide sequencing of the PCR products revealed nine different variable regions. One resistance gene was identified in five variable regions (aadA1, aadA2, aadA23, dfrV and pse-1), and four variable regions carried two resistance gene cassettes (aadB-catB3, dhfrI-aadA, dfrA17-aadA5 and oxa-1-aadA1).",
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T1 - Antibiotic resistance and class 1 integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003

AU - Nógrády, Noémi

AU - Gadó, István

AU - Tóth, A.

AU - Pászti, J.

PY - 2005/8

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N2 - The antibiotic resistance profiles of 5178 Salmonella strains representing 19 non-typhoidal serotypes isolated from human salmonellosis cases in Hungary in 2002 and 2003 were analysed for resistance to 10 antibiotic agents. The most frequent resistances were to nalidixic acid (Nx), streptomycin (S), tetracycline (Tc), ampicillin (Amp) and chloramphenicol (Cm) (ranging from 27% to 13%). Forty-five percent of the Salmonella Typhimurium strains were multiple resistant and belonged mainly to the definitive phage types 104 and U302. A prevalence of 83-94% of strains of serotypes S. Infantis, S. Hadar and S. Virchow was observed with the NxSTc resistance pattern, sometimes complemented with other resistances. Multiple resistance was uncommon in S. Enteritidis; nevertheless, 20% of the strains, most of which belonged to phage type 4, were nalidixic acid resistant. One strain of S. Typhimurium was found to be resistant to ciprofloxacin. Four S. Typhimurium strains were resistant to cefotaxime and produced extended-spectrum β-lactamase. Selected isolates were screened for the presence of class 1 integrons by polymerase chain reaction (PCR). Nucleotide sequencing of the PCR products revealed nine different variable regions. One resistance gene was identified in five variable regions (aadA1, aadA2, aadA23, dfrV and pse-1), and four variable regions carried two resistance gene cassettes (aadB-catB3, dhfrI-aadA, dfrA17-aadA5 and oxa-1-aadA1).

AB - The antibiotic resistance profiles of 5178 Salmonella strains representing 19 non-typhoidal serotypes isolated from human salmonellosis cases in Hungary in 2002 and 2003 were analysed for resistance to 10 antibiotic agents. The most frequent resistances were to nalidixic acid (Nx), streptomycin (S), tetracycline (Tc), ampicillin (Amp) and chloramphenicol (Cm) (ranging from 27% to 13%). Forty-five percent of the Salmonella Typhimurium strains were multiple resistant and belonged mainly to the definitive phage types 104 and U302. A prevalence of 83-94% of strains of serotypes S. Infantis, S. Hadar and S. Virchow was observed with the NxSTc resistance pattern, sometimes complemented with other resistances. Multiple resistance was uncommon in S. Enteritidis; nevertheless, 20% of the strains, most of which belonged to phage type 4, were nalidixic acid resistant. One strain of S. Typhimurium was found to be resistant to ciprofloxacin. Four S. Typhimurium strains were resistant to cefotaxime and produced extended-spectrum β-lactamase. Selected isolates were screened for the presence of class 1 integrons by polymerase chain reaction (PCR). Nucleotide sequencing of the PCR products revealed nine different variable regions. One resistance gene was identified in five variable regions (aadA1, aadA2, aadA23, dfrV and pse-1), and four variable regions carried two resistance gene cassettes (aadB-catB3, dhfrI-aadA, dfrA17-aadA5 and oxa-1-aadA1).

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