Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA

Andrea Németh, Norbert Orgovan, Barbara W. Sódar, Xabier Osteikoetxea, Krisztina Pálóczi, K. Szabó, Krisztina V. Vukman, A. Kittel, Lilla Turiák, Zoltán Wiener, S. Tóth, L. Drahos, K. Vékey, R. Horváth, E. Búzás

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes.

Original languageEnglish
Article number8202
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Exosomes
Ciprofloxacin
Anti-Bacterial Agents
DNA
Mycoplasma
Fluoroquinolones
DNA-Binding Proteins
Biosensing Techniques
Mitochondrial DNA
Fibronectins
Bacterial Infections
Oxidative Stress
Cell Culture Techniques
Extracellular Vesicles
Apoptosis

ASJC Scopus subject areas

  • General

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Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. / Németh, Andrea; Orgovan, Norbert; Sódar, Barbara W.; Osteikoetxea, Xabier; Pálóczi, Krisztina; Szabó, K.; Vukman, Krisztina V.; Kittel, A.; Turiák, Lilla; Wiener, Zoltán; Tóth, S.; Drahos, L.; Vékey, K.; Horváth, R.; Búzás, E.

In: Scientific Reports, Vol. 7, No. 1, 8202, 01.12.2017.

Research output: Contribution to journalArticle

Németh, Andrea ; Orgovan, Norbert ; Sódar, Barbara W. ; Osteikoetxea, Xabier ; Pálóczi, Krisztina ; Szabó, K. ; Vukman, Krisztina V. ; Kittel, A. ; Turiák, Lilla ; Wiener, Zoltán ; Tóth, S. ; Drahos, L. ; Vékey, K. ; Horváth, R. ; Búzás, E. / Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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