Antibiosis, antibiotics and the formaldehyde cycle: The unique importance of planar chromatographic techniques to progress in these fields

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The BioArena system, which integrates the up-to-date methodology and biological results of bioautography with OPLC as an efficient planar separation technique (compact spots, etc.), is especially suitable for investigating biochemical interactions in an adsorbent bed after chromatographic separation. The first results from BioArena show that formaldehyde (HCHO), which can originate from pathogen cells in some situations, can play a special role in the antibiotic activity of trans-resveratrol. When L-arginine and glutathione were used as endogenous HCHO-capturing molecules in the culture medium the antimicrobial activity of trans-resveratrol on the adsorbent layer decreased substantially. It has been observed that trans-resveratrol generates a time-dependent, and therefore concentration-dependent, duplicate inhibiting effect on the pathogen, and that the BioArena system was suitable for illustration of this new phenomenon. It is probable that this effect occurs as a result of HCHO, with special emphasis on the possibility of interaction between the HCHO and H2O2 of endogenous origin on the adsorbent layer incubated with pathogen cells. It seems that the BioArena system will be an important, indispensable complement to the basic separation technique OPLC.

Original languageEnglish
Pages (from-to)84-88
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume17
Issue number2
DOIs
Publication statusPublished - Mar 1 2004

Keywords

  • Antibiosis
  • Antibiotics
  • BioArena
  • Bioautography
  • Formaldehyde cycle
  • OPLC
  • TLC/HPTLC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Antibiosis, antibiotics and the formaldehyde cycle: The unique importance of planar chromatographic techniques to progress in these fields'. Together they form a unique fingerprint.

  • Cite this