Antibacterial and resistance modifying activities of nigella sativa essential oil and its active compounds against listeria monocytogenes

Ahmad Mouwakeh, Ágnes Telbisz, G. Spengler, Csilla Mohácsi-Farkas, Gabriella Kiskó

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background/Aim: N. sativa essential oil (EO) and its compounds (thymoquinone, carvacrol and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol and p-cymene against Listeria monocytogenes. Materials and Methods: N. sativa EO, thymoquinone, carvacrol and p-cymene was assessed for its antimicrobial activity, modulation of antimicrobial resistance, inhibition of antimicrobial efflux and membrane integrity by broth microdilution, ethidium bromide accumulation and LIVE/DEAD BacLight™ assays. Results: L. monocytogenes showed substantial susceptibility toward N. sativa EO, thymoquinone, and carvacrol. A significant reduction in MIC’s of EtBr and ciprofloxacin was noticed when tested in combination with N. sativa EO, thymoquinone, carvacrol and reserpine. In the presence of each compound the membrane integrity was disintegrated, and the EtBr accumulation increased which was comparable to positive control reserpine. Conclusion: N. sativa EO might have a potential for controlling the antibiotic resistance in Listeria.

Original languageEnglish
Pages (from-to)737-743
Number of pages7
JournalIn Vivo
Volume32
Issue number4
DOIs
Publication statusPublished - Jul 1 2018

Fingerprint

Nigella sativa
Listeria
Listeria monocytogenes
Volatile Oils
Reserpine
Membranes
Ethidium
Ciprofloxacin
Microbial Drug Resistance
caraway oil
Assays
Modulation
carvacrol
thymoquinone
Anti-Bacterial Agents

Keywords

  • Antimicrobial activity
  • Listeria monocytogenes
  • N. sativa essential oil
  • Resistance modifying activity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Antibacterial and resistance modifying activities of nigella sativa essential oil and its active compounds against listeria monocytogenes. / Mouwakeh, Ahmad; Telbisz, Ágnes; Spengler, G.; Mohácsi-Farkas, Csilla; Kiskó, Gabriella.

In: In Vivo, Vol. 32, No. 4, 01.07.2018, p. 737-743.

Research output: Contribution to journalArticle

Mouwakeh, Ahmad ; Telbisz, Ágnes ; Spengler, G. ; Mohácsi-Farkas, Csilla ; Kiskó, Gabriella. / Antibacterial and resistance modifying activities of nigella sativa essential oil and its active compounds against listeria monocytogenes. In: In Vivo. 2018 ; Vol. 32, No. 4. pp. 737-743.
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