Antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (Beta vulgaris L.)

Ottó Toidi, Gábor Gyulai, József Kiss, Imre A. Tamás, Ervin Balázs

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An in vitro method was developed for microshoot initiation from thin- layer explants prepared from the elongated epicotyls of sugarbeet (Beta vulgaris L.). Intact epicotyls of 14-day-old seedlings were excised from the hypocotyls above the cotyledons and allowed to elongate on De Greef and Jacobs (1979) medium supplemented with 0.2 mg/l 6benzyladenine, 0.2 mg/l gibberellic acid and 0.1 mg/l indole-3-acetic acid in darkness. After a 21- day-incubation, the elongated epicotyls were halved to obtain apical and basal segments prior to removing the leaves and lateral buds. Subsequently, 5-8 mm long, 2-3 mm wide and 0.81.0 mm thick tangential sections were prepared longitudinally from the exterior parts of the halved epicotyls. These thin-layer explants were incubated on microshoot initiating media containing various growth regulators. The combination of 1.0 mg/l 6- benzyladenine and the antiauxin 2,3,5-triiodobenzoic acid (1.0 mg/l) resulted in maximum microshoot development (6.3±0.2 microshoots/thin layer explant). The final efficiency of our tissue culture system was significantly increased by the NaCl (100 mg/l) initiated in vitro rooting of microshoot originated plantlets.

Original languageEnglish
Pages (from-to)851-854
Number of pages4
JournalPlant Cell Reports
Volume15
Issue number11
DOIs
Publication statusPublished - Jan 1 1996

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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