Antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (Beta vulgaris L.)

O. Toldi, Gábor Gyulai, J. Kiss, Imre A. Tamás, E. Balázs

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An in vitro method was developed for microshoot initiation from thin-layer explants prepared from the elongated epicotyls of sugarbeet (Beta vulgaris L.). Intact epicotyls of 14-day-old seedlings were excised from the hypocotyls above the cotyledons and allowed to elongate on De Greef and Jacobs (1979) medium supplemented with 0.2 mg/l 6-benzyladenine, 0.2 mg/l gibberellic acid and 0.1 mg/l indole-3-acetic acid in darkness. After a 21-day-incubation, the elongated epicotyls were halved to obtain apical and basal segments prior to removing the leaves and lateral buds. Subsequently, 5-8 mm long, 2-3 mm wide and 0.8-1.0 mm thick tangential sections were prepared longitudinally from the exterior parts of the halved epicotyls. These thin-layer explants were incubated on microshoot initiating media containing various growth regulators. The combination of 1.0 mg/l 6-benzyladenine and the antiauxin 2,3,5-triiodobenzoic acid (1.0 mg/l) resulted in maximum microshoot development (6.3±0.2 microshoots/thin-layer explant). The final efficiency of our tissue culture system was significantly increased by the NaCl (100 mg/l) initiated in vitro rooting of microshoot originated plantlets.

Original languageEnglish
Pages (from-to)851-854
Number of pages4
JournalPlant Cell Reports
Volume15
Issue number11
DOIs
Publication statusPublished - Aug 1996

Fingerprint

epicotyls
Beta vulgaris
sugar beet
explants
benzyladenine
growth regulators
gibberellic acid
indole acetic acid
hypocotyls
tissue culture
cotyledons
plantlets
rooting
buds
seedlings
acids
leaves
methodology

ASJC Scopus subject areas

  • Plant Science

Cite this

Antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (Beta vulgaris L.). / Toldi, O.; Gyulai, Gábor; Kiss, J.; Tamás, Imre A.; Balázs, E.

In: Plant Cell Reports, Vol. 15, No. 11, 08.1996, p. 851-854.

Research output: Contribution to journalArticle

@article{738a5f49cb6a409c9208cf21d8408bd9,
title = "Antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (Beta vulgaris L.)",
abstract = "An in vitro method was developed for microshoot initiation from thin-layer explants prepared from the elongated epicotyls of sugarbeet (Beta vulgaris L.). Intact epicotyls of 14-day-old seedlings were excised from the hypocotyls above the cotyledons and allowed to elongate on De Greef and Jacobs (1979) medium supplemented with 0.2 mg/l 6-benzyladenine, 0.2 mg/l gibberellic acid and 0.1 mg/l indole-3-acetic acid in darkness. After a 21-day-incubation, the elongated epicotyls were halved to obtain apical and basal segments prior to removing the leaves and lateral buds. Subsequently, 5-8 mm long, 2-3 mm wide and 0.8-1.0 mm thick tangential sections were prepared longitudinally from the exterior parts of the halved epicotyls. These thin-layer explants were incubated on microshoot initiating media containing various growth regulators. The combination of 1.0 mg/l 6-benzyladenine and the antiauxin 2,3,5-triiodobenzoic acid (1.0 mg/l) resulted in maximum microshoot development (6.3±0.2 microshoots/thin-layer explant). The final efficiency of our tissue culture system was significantly increased by the NaCl (100 mg/l) initiated in vitro rooting of microshoot originated plantlets.",
author = "O. Toldi and G{\'a}bor Gyulai and J. Kiss and Tam{\'a}s, {Imre A.} and E. Bal{\'a}zs",
year = "1996",
month = "8",
doi = "10.1007/BF00233155",
language = "English",
volume = "15",
pages = "851--854",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "11",

}

TY - JOUR

T1 - Antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (Beta vulgaris L.)

AU - Toldi, O.

AU - Gyulai, Gábor

AU - Kiss, J.

AU - Tamás, Imre A.

AU - Balázs, E.

PY - 1996/8

Y1 - 1996/8

N2 - An in vitro method was developed for microshoot initiation from thin-layer explants prepared from the elongated epicotyls of sugarbeet (Beta vulgaris L.). Intact epicotyls of 14-day-old seedlings were excised from the hypocotyls above the cotyledons and allowed to elongate on De Greef and Jacobs (1979) medium supplemented with 0.2 mg/l 6-benzyladenine, 0.2 mg/l gibberellic acid and 0.1 mg/l indole-3-acetic acid in darkness. After a 21-day-incubation, the elongated epicotyls were halved to obtain apical and basal segments prior to removing the leaves and lateral buds. Subsequently, 5-8 mm long, 2-3 mm wide and 0.8-1.0 mm thick tangential sections were prepared longitudinally from the exterior parts of the halved epicotyls. These thin-layer explants were incubated on microshoot initiating media containing various growth regulators. The combination of 1.0 mg/l 6-benzyladenine and the antiauxin 2,3,5-triiodobenzoic acid (1.0 mg/l) resulted in maximum microshoot development (6.3±0.2 microshoots/thin-layer explant). The final efficiency of our tissue culture system was significantly increased by the NaCl (100 mg/l) initiated in vitro rooting of microshoot originated plantlets.

AB - An in vitro method was developed for microshoot initiation from thin-layer explants prepared from the elongated epicotyls of sugarbeet (Beta vulgaris L.). Intact epicotyls of 14-day-old seedlings were excised from the hypocotyls above the cotyledons and allowed to elongate on De Greef and Jacobs (1979) medium supplemented with 0.2 mg/l 6-benzyladenine, 0.2 mg/l gibberellic acid and 0.1 mg/l indole-3-acetic acid in darkness. After a 21-day-incubation, the elongated epicotyls were halved to obtain apical and basal segments prior to removing the leaves and lateral buds. Subsequently, 5-8 mm long, 2-3 mm wide and 0.8-1.0 mm thick tangential sections were prepared longitudinally from the exterior parts of the halved epicotyls. These thin-layer explants were incubated on microshoot initiating media containing various growth regulators. The combination of 1.0 mg/l 6-benzyladenine and the antiauxin 2,3,5-triiodobenzoic acid (1.0 mg/l) resulted in maximum microshoot development (6.3±0.2 microshoots/thin-layer explant). The final efficiency of our tissue culture system was significantly increased by the NaCl (100 mg/l) initiated in vitro rooting of microshoot originated plantlets.

UR - http://www.scopus.com/inward/record.url?scp=0029744060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029744060&partnerID=8YFLogxK

U2 - 10.1007/BF00233155

DO - 10.1007/BF00233155

M3 - Article

VL - 15

SP - 851

EP - 854

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 11

ER -