Anti-inflammatory effect of recreational exercise in TNBS-Induced colitis in rats: Role of NOS/HO/MPO system

Zita Szalai, András Szász, István Nagy, L. Puskás, Krisztina Kupai, Adél Király, A. Berkó, A. Pósa, Gerda Strifler, Zoltán Baráth, Lajos I. Nagy, Renáta Szabó, Imre Pávó, Zsolt Murlasits, Mariann Gyöngyösi, C. Varga

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

There are opposite views in the available literature: Whether physical exercise has a protective effect or not on the onset of inflammatory bowel disease (IBD). Therefore, we investigated the effects of recreational physical exercise before the induction of colitis. After 6 weeks of voluntary physical activity (running wheel), male Wistar rats were treated with TNBS (10 mg). 72 hrs after trinitrobenzene sulphonic acid (TNBS) challenge we measured colonic gene (TNF-α, IL-1β, CXCL1 and IL-10) and protein (TNF-α) expressions of various inflammatory mediators and enzyme activities of heme oxygenase (HO), nitric oxide synthase (NOS), and myeloperoxidase (MPO) enzymes. Wheel running significantly increased the activities of HO, constitutive NOS (cNOS) isoform. Furthermore, 6 weeks of running significantly decreased TNBS-induced inflammatory markers, including extent of lesions, severity of mucosal damage, and gene expression of IL-1β, CXCL1, and MPO activity, while IL-10 gene expression and cNOS activity were increased. iNOS activity decreased and the activity of HO enzyme increased, but not significantly, compared to the sedentary TNBS-treated group. In conclusion, recreational physical exercise can play an anti-inflammatory role by downregulating the gene expression of proinflammatory mediators, inducing anti-inflammatory mediators, and modulating the activities of HO and NOS enzymes in a rat model of colitis.

Original languageEnglish
Article number925981
JournalOxidative Medicine and Cellular Longevity
Volume2014
DOIs
Publication statusPublished - 2014

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Trinitrobenzenes
Heme Oxygenase (Decyclizing)
Sulfonic Acids
Colitis
Nitric Oxide Synthase
Peroxidase
Rats
Anti-Inflammatory Agents
Gene expression
Running
Exercise
Enzymes
Interleukin-1
Gene Expression
Interleukin-10
Wheels
Enzyme activity
Inflammatory Bowel Diseases
Wistar Rats
Protein Isoforms

ASJC Scopus subject areas

  • Cell Biology
  • Ageing
  • Biochemistry
  • Medicine(all)

Cite this

Anti-inflammatory effect of recreational exercise in TNBS-Induced colitis in rats : Role of NOS/HO/MPO system. / Szalai, Zita; Szász, András; Nagy, István; Puskás, L.; Kupai, Krisztina; Király, Adél; Berkó, A.; Pósa, A.; Strifler, Gerda; Baráth, Zoltán; Nagy, Lajos I.; Szabó, Renáta; Pávó, Imre; Murlasits, Zsolt; Gyöngyösi, Mariann; Varga, C.

In: Oxidative Medicine and Cellular Longevity, Vol. 2014, 925981, 2014.

Research output: Contribution to journalArticle

Szalai, Z, Szász, A, Nagy, I, Puskás, L, Kupai, K, Király, A, Berkó, A, Pósa, A, Strifler, G, Baráth, Z, Nagy, LI, Szabó, R, Pávó, I, Murlasits, Z, Gyöngyösi, M & Varga, C 2014, 'Anti-inflammatory effect of recreational exercise in TNBS-Induced colitis in rats: Role of NOS/HO/MPO system', Oxidative Medicine and Cellular Longevity, vol. 2014, 925981. https://doi.org/10.1155/2014/925981
Szalai, Zita ; Szász, András ; Nagy, István ; Puskás, L. ; Kupai, Krisztina ; Király, Adél ; Berkó, A. ; Pósa, A. ; Strifler, Gerda ; Baráth, Zoltán ; Nagy, Lajos I. ; Szabó, Renáta ; Pávó, Imre ; Murlasits, Zsolt ; Gyöngyösi, Mariann ; Varga, C. / Anti-inflammatory effect of recreational exercise in TNBS-Induced colitis in rats : Role of NOS/HO/MPO system. In: Oxidative Medicine and Cellular Longevity. 2014 ; Vol. 2014.
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abstract = "There are opposite views in the available literature: Whether physical exercise has a protective effect or not on the onset of inflammatory bowel disease (IBD). Therefore, we investigated the effects of recreational physical exercise before the induction of colitis. After 6 weeks of voluntary physical activity (running wheel), male Wistar rats were treated with TNBS (10 mg). 72 hrs after trinitrobenzene sulphonic acid (TNBS) challenge we measured colonic gene (TNF-α, IL-1β, CXCL1 and IL-10) and protein (TNF-α) expressions of various inflammatory mediators and enzyme activities of heme oxygenase (HO), nitric oxide synthase (NOS), and myeloperoxidase (MPO) enzymes. Wheel running significantly increased the activities of HO, constitutive NOS (cNOS) isoform. Furthermore, 6 weeks of running significantly decreased TNBS-induced inflammatory markers, including extent of lesions, severity of mucosal damage, and gene expression of IL-1β, CXCL1, and MPO activity, while IL-10 gene expression and cNOS activity were increased. iNOS activity decreased and the activity of HO enzyme increased, but not significantly, compared to the sedentary TNBS-treated group. In conclusion, recreational physical exercise can play an anti-inflammatory role by downregulating the gene expression of proinflammatory mediators, inducing anti-inflammatory mediators, and modulating the activities of HO and NOS enzymes in a rat model of colitis.",
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AU - Nagy, István

AU - Puskás, L.

AU - Kupai, Krisztina

AU - Király, Adél

AU - Berkó, A.

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AU - Nagy, Lajos I.

AU - Szabó, Renáta

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