Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds

Erzsébet Háznagy-Radnai, Laura Fási, Edit Wéber, Gyula Pinke, Gergely Király, Anita Sztojkov-Ivanov, R. Gáspár, J. Hohmann

Research output: Contribution to journalArticle

Abstract

Melampyrum barbatum Waldst. & Kit. Ex Willd. (Scrophulariaceae) has been used in traditional medicine for the treatment of rheumatic complaints and different skin diseases. In the course of our study the anti-inflammatory activity of the aerial parts of M. Barbatum was evaluated. A MeOH extract was prepared and consecutively partitioned with CHCl3, EtOAc and n-BuOH. The fractions were assayed in in vivo carrageenan-induced rat paw oedema model. The intraperitoneally administered n-BuOH phase exerted marked inhibitory effect (33.6 %, p < 0.01). Multistep chromatographic separation afforded mussaenoside and aucubine from n-BuOH fraction. Moreover, 8-epiloganin, loganic acid and mussaenoside were obtained from EtOAc fraction and apigenin, luteolin, benzoic acid and galactitol from CHCl3 fraction. These data validate the ethnomedicinal use of M. Barbatum for the treatment of inflammatory diseases and reveal that iridoids and flavonoids could be responsible for the anti-inflammatory effect of this species.

Original languageEnglish
Pages (from-to)235-236
Number of pages2
JournalNatural Product Communications
Volume13
Issue number3
Publication statusPublished - Jan 1 2018

Fingerprint

Melampyrum
anti-inflammatory activity
Flavonoids
Anti-Inflammatory Agents
flavonoids
Scrophulariaceae
Galactitol
Iridoids
galactitol
Apigenin
Luteolin
iridoids
Benzoic Acid
apigenin
Carrageenan
benzoic acid
luteolin
carrageenan
skin diseases
Traditional Medicine

Keywords

  • Anti-inflammatory activity.
  • Flavonoids
  • Iridoids
  • Melampyrum barbatum
  • Scrophulariaceae

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Plant Science
  • Complementary and alternative medicine

Cite this

Háznagy-Radnai, E., Fási, L., Wéber, E., Pinke, G., Király, G., Sztojkov-Ivanov, A., ... Hohmann, J. (2018). Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds. Natural Product Communications, 13(3), 235-236.

Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds. / Háznagy-Radnai, Erzsébet; Fási, Laura; Wéber, Edit; Pinke, Gyula; Király, Gergely; Sztojkov-Ivanov, Anita; Gáspár, R.; Hohmann, J.

In: Natural Product Communications, Vol. 13, No. 3, 01.01.2018, p. 235-236.

Research output: Contribution to journalArticle

Háznagy-Radnai, E, Fási, L, Wéber, E, Pinke, G, Király, G, Sztojkov-Ivanov, A, Gáspár, R & Hohmann, J 2018, 'Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds', Natural Product Communications, vol. 13, no. 3, pp. 235-236.
Háznagy-Radnai E, Fási L, Wéber E, Pinke G, Király G, Sztojkov-Ivanov A et al. Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds. Natural Product Communications. 2018 Jan 1;13(3):235-236.
Háznagy-Radnai, Erzsébet ; Fási, Laura ; Wéber, Edit ; Pinke, Gyula ; Király, Gergely ; Sztojkov-Ivanov, Anita ; Gáspár, R. ; Hohmann, J. / Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds. In: Natural Product Communications. 2018 ; Vol. 13, No. 3. pp. 235-236.
@article{20a4d0b9381d4b588fdadb8351205d24,
title = "Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds",
abstract = "Melampyrum barbatum Waldst. & Kit. Ex Willd. (Scrophulariaceae) has been used in traditional medicine for the treatment of rheumatic complaints and different skin diseases. In the course of our study the anti-inflammatory activity of the aerial parts of M. Barbatum was evaluated. A MeOH extract was prepared and consecutively partitioned with CHCl3, EtOAc and n-BuOH. The fractions were assayed in in vivo carrageenan-induced rat paw oedema model. The intraperitoneally administered n-BuOH phase exerted marked inhibitory effect (33.6 {\%}, p < 0.01). Multistep chromatographic separation afforded mussaenoside and aucubine from n-BuOH fraction. Moreover, 8-epiloganin, loganic acid and mussaenoside were obtained from EtOAc fraction and apigenin, luteolin, benzoic acid and galactitol from CHCl3 fraction. These data validate the ethnomedicinal use of M. Barbatum for the treatment of inflammatory diseases and reveal that iridoids and flavonoids could be responsible for the anti-inflammatory effect of this species.",
keywords = "Anti-inflammatory activity., Flavonoids, Iridoids, Melampyrum barbatum, Scrophulariaceae",
author = "Erzs{\'e}bet H{\'a}znagy-Radnai and Laura F{\'a}si and Edit W{\'e}ber and Gyula Pinke and Gergely Kir{\'a}ly and Anita Sztojkov-Ivanov and R. G{\'a}sp{\'a}r and J. Hohmann",
year = "2018",
month = "1",
day = "1",
language = "English",
volume = "13",
pages = "235--236",
journal = "Natural Product Communications",
issn = "1934-578X",
publisher = "Natural Product Communications",
number = "3",

}

TY - JOUR

T1 - Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds

AU - Háznagy-Radnai, Erzsébet

AU - Fási, Laura

AU - Wéber, Edit

AU - Pinke, Gyula

AU - Király, Gergely

AU - Sztojkov-Ivanov, Anita

AU - Gáspár, R.

AU - Hohmann, J.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Melampyrum barbatum Waldst. & Kit. Ex Willd. (Scrophulariaceae) has been used in traditional medicine for the treatment of rheumatic complaints and different skin diseases. In the course of our study the anti-inflammatory activity of the aerial parts of M. Barbatum was evaluated. A MeOH extract was prepared and consecutively partitioned with CHCl3, EtOAc and n-BuOH. The fractions were assayed in in vivo carrageenan-induced rat paw oedema model. The intraperitoneally administered n-BuOH phase exerted marked inhibitory effect (33.6 %, p < 0.01). Multistep chromatographic separation afforded mussaenoside and aucubine from n-BuOH fraction. Moreover, 8-epiloganin, loganic acid and mussaenoside were obtained from EtOAc fraction and apigenin, luteolin, benzoic acid and galactitol from CHCl3 fraction. These data validate the ethnomedicinal use of M. Barbatum for the treatment of inflammatory diseases and reveal that iridoids and flavonoids could be responsible for the anti-inflammatory effect of this species.

AB - Melampyrum barbatum Waldst. & Kit. Ex Willd. (Scrophulariaceae) has been used in traditional medicine for the treatment of rheumatic complaints and different skin diseases. In the course of our study the anti-inflammatory activity of the aerial parts of M. Barbatum was evaluated. A MeOH extract was prepared and consecutively partitioned with CHCl3, EtOAc and n-BuOH. The fractions were assayed in in vivo carrageenan-induced rat paw oedema model. The intraperitoneally administered n-BuOH phase exerted marked inhibitory effect (33.6 %, p < 0.01). Multistep chromatographic separation afforded mussaenoside and aucubine from n-BuOH fraction. Moreover, 8-epiloganin, loganic acid and mussaenoside were obtained from EtOAc fraction and apigenin, luteolin, benzoic acid and galactitol from CHCl3 fraction. These data validate the ethnomedicinal use of M. Barbatum for the treatment of inflammatory diseases and reveal that iridoids and flavonoids could be responsible for the anti-inflammatory effect of this species.

KW - Anti-inflammatory activity.

KW - Flavonoids

KW - Iridoids

KW - Melampyrum barbatum

KW - Scrophulariaceae

UR - http://www.scopus.com/inward/record.url?scp=85048153643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048153643&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85048153643

VL - 13

SP - 235

EP - 236

JO - Natural Product Communications

JF - Natural Product Communications

SN - 1934-578X

IS - 3

ER -