Anti-haemophilus activity of selected essential oils detected by TLC-direct bioautography and biofilm inhibition

Viktória Lilla Balázs, Barbara Horváth, Erika Kerekes, Kamilla Ács, Béla Kocsis, Adorján Varga, Andrea Böszörményi, Dávid U. Nagy, Judit Krisch, Aleksandar Széchenyi, G. Horváth

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Haemophilus influenzae and H. parainfluenzae. Oil in water (O/W) type Pickering nano-emulsions stabilized with silica nanoparticles from each oil were prepared to increase their water-solubility. Samples with Tween80 surfactant and absolute ethanol were also used. Results showed that H. influenzae was more sensitive to the EOs than H. parainfluenzae (except for cinnamon bark oil). In thin layer chromatography-direct bioautography (TLC-DB) the ethanolic solutions of thyme oil presented the best activity against H. influenzae, while cinnamon oil was the most active against H. parainfluenzae. Pickering nano-emulsion of cinnamon oil inhibited the biofilm formation of H. parainfluenzae (76.35%) more efficiently than samples with Tween80 surfactant or absolute ethanol. In conclusion, Pickering nano-emulsion of EOs could inhibit the biofilm production effectively.

Original languageEnglish
Article number3301
JournalMolecules
Volume24
Issue number18
DOIs
Publication statusPublished - Sep 11 2019

Fingerprint

thin layer chromatography
Haemophilus
Thin layer chromatography
biofilms
Biofilms
Volatile Oils
Paramyxoviridae Infections
Thin Layer Chromatography
Cinnamomum zeylanicum
Oils
oils
Haemophilus influenzae
Emulsions
Thymus Plant
Surface-Active Agents
influenza
Ethanol
Mentha piperita
Syzygium
emulsions

Keywords

  • Anti-biofilm activity
  • Cinnamon bark
  • Clove
  • Essential oil
  • Haemophilus influenza
  • Haemophilus parainfluenzae
  • Peppermint
  • Pickering nano-emulsion
  • Thyme

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Anti-haemophilus activity of selected essential oils detected by TLC-direct bioautography and biofilm inhibition. / Balázs, Viktória Lilla; Horváth, Barbara; Kerekes, Erika; Ács, Kamilla; Kocsis, Béla; Varga, Adorján; Böszörményi, Andrea; Nagy, Dávid U.; Krisch, Judit; Széchenyi, Aleksandar; Horváth, G.

In: Molecules, Vol. 24, No. 18, 3301, 11.09.2019.

Research output: Contribution to journalArticle

Balázs, VL, Horváth, B, Kerekes, E, Ács, K, Kocsis, B, Varga, A, Böszörményi, A, Nagy, DU, Krisch, J, Széchenyi, A & Horváth, G 2019, 'Anti-haemophilus activity of selected essential oils detected by TLC-direct bioautography and biofilm inhibition', Molecules, vol. 24, no. 18, 3301. https://doi.org/10.3390/molecules24183301
Balázs, Viktória Lilla ; Horváth, Barbara ; Kerekes, Erika ; Ács, Kamilla ; Kocsis, Béla ; Varga, Adorján ; Böszörményi, Andrea ; Nagy, Dávid U. ; Krisch, Judit ; Széchenyi, Aleksandar ; Horváth, G. / Anti-haemophilus activity of selected essential oils detected by TLC-direct bioautography and biofilm inhibition. In: Molecules. 2019 ; Vol. 24, No. 18.
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abstract = "Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Haemophilus influenzae and H. parainfluenzae. Oil in water (O/W) type Pickering nano-emulsions stabilized with silica nanoparticles from each oil were prepared to increase their water-solubility. Samples with Tween80 surfactant and absolute ethanol were also used. Results showed that H. influenzae was more sensitive to the EOs than H. parainfluenzae (except for cinnamon bark oil). In thin layer chromatography-direct bioautography (TLC-DB) the ethanolic solutions of thyme oil presented the best activity against H. influenzae, while cinnamon oil was the most active against H. parainfluenzae. Pickering nano-emulsion of cinnamon oil inhibited the biofilm formation of H. parainfluenzae (76.35{\%}) more efficiently than samples with Tween80 surfactant or absolute ethanol. In conclusion, Pickering nano-emulsion of EOs could inhibit the biofilm production effectively.",
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AU - Ács, Kamilla

AU - Kocsis, Béla

AU - Varga, Adorján

AU - Böszörményi, Andrea

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