Anthocyanin-rich black currant (Ribes nigrum L.) extract affords chemoprevention against diethylnitrosamine-induced hepatocellular carcinogenesis in rats

Anupam Bishayee, Thomas Mbimba, Roslin J. Thoppil, Erzsébet Háznagy-Radnai, P. Sípos, Altaf S. Darvesh, Hans G. Folkesson, J. Hohmann

Research output: Contribution to journalArticle

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Abstract

Anthocyanins are known to possess potent anticarcinogenic properties against several cancers thus demonstrating potential for cancer prevention. Black currant (Ribes nigrum L., Grossulariaceae) fruits have a high anthocyanin content. This "superfruit" is known to possess various pharmacological effects including alleviation of chronic oxidative stress and inflammation. In contrast to a large volume of literature on the health benefits of black currant, limited evidence on antitumor effects of black currant exists with virtually no data on the prevention of experimental carcinogenesis. In the current study, we have investigated the chemopreventive effects of an anthocyanin-rich black currant skin extract (BCSE) utilizing our well-characterized model of rat liver carcinogenesis. Initiation of hepatocarcinogenesis was done by intraperitoneal injection of diethylnitrosamine (DENA) followed by promotion with phenobarbital. The rats were exposed to dietary BCSE for 4 weeks prior to initiation, and the treatment was continued for 22 consecutive weeks. BCSE dose-dependently decreased the incidence, total number, multiplicity, size and volume of preneoplastic hepatic nodules. The antihepatocarcinogenic effect of BCSE was confirmed by histopathological examination of liver sections. Immunohistochemical analysis of proliferating cell nuclear antigen and DNA fragmentation revealed BCSE-mediated inhibition of abnormal cell proliferation and induction of apoptosis in DENA-induced rat liver tumorigenesis respectively. Mechanistic studies revealed that BCSE-mediated proapototic signal during experimental hepatocarcinogenesis may be propagated via the up-regulation of Bax and down-regulation of Bcl-2 expression at the translational level. These results along with a safety profile of BCSE encourage the development of black currant bioactive constituents as chemopreventive agents for human liver cancer.

Original languageEnglish
Pages (from-to)1035-1046
Number of pages12
JournalJournal of Nutritional Biochemistry
Volume22
Issue number11
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Ribes
Diethylnitrosamine
Anthocyanins
Chemoprevention
Rats
Skin
Carcinogenesis
Liver
Grossulariaceae
Oxidative stress
Proliferating Cell Nuclear Antigen
Cell proliferation
Phenobarbital
Fruits
Health
Apoptosis
Insurance Benefits
DNA Fragmentation
Liver Neoplasms
Intraperitoneal Injections

Keywords

  • Apoptosis
  • Black currant
  • Cell proliferation
  • Chemoprevention
  • Diethylnitrosamine
  • Hepatocarcinogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Anthocyanin-rich black currant (Ribes nigrum L.) extract affords chemoprevention against diethylnitrosamine-induced hepatocellular carcinogenesis in rats. / Bishayee, Anupam; Mbimba, Thomas; Thoppil, Roslin J.; Háznagy-Radnai, Erzsébet; Sípos, P.; Darvesh, Altaf S.; Folkesson, Hans G.; Hohmann, J.

In: Journal of Nutritional Biochemistry, Vol. 22, No. 11, 11.2011, p. 1035-1046.

Research output: Contribution to journalArticle

Bishayee, Anupam ; Mbimba, Thomas ; Thoppil, Roslin J. ; Háznagy-Radnai, Erzsébet ; Sípos, P. ; Darvesh, Altaf S. ; Folkesson, Hans G. ; Hohmann, J. / Anthocyanin-rich black currant (Ribes nigrum L.) extract affords chemoprevention against diethylnitrosamine-induced hepatocellular carcinogenesis in rats. In: Journal of Nutritional Biochemistry. 2011 ; Vol. 22, No. 11. pp. 1035-1046.
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