Antagonistic interaction between MAP kinase and protein phosphatase 2C in stress recovery

Jeffrey Leung, Sofia Orfanidi, Françoise Chefdor, T. Mészáros, Susanne Bolte, Tsuyoshi Mizoguchi, Kazuo Shinozaki, Jérôme Giraudat, Laszlo Bögre

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The Arabidopsis mitogen-activated kinase MPK6 transmits a diversity of stress signals. However, much less is known about resetting mechanisms subsequent to the stress response. We show that MPK6 is a potential target of abscisic acid-insensitive 1 (ABI1), a protein phosphatase 2C that acts as a key element in attenuating abscisic acid (ABA)-dependent stress signaling. MPK6 can bind to ABI1 in vitro and in yeast, and that a complex containing these two proteins can be co-precipitated from transfected Arabidopsis cells. In whole plants, MPK6 is hyper-reactive to osmotic stress in mutants compromised in ABI1 activity, but not in its closest functional homolog ABI2. Moreover, conditional expression of a dominant-negative form of mpk6 renders plants hypersensitive to ABA. MPK6 enhances the synthesis of ethylene [1]. ABI1 may therefore down modulate ethylene as part of the resetting mechanism after stress.

Original languageEnglish
Pages (from-to)596-606
Number of pages11
JournalPlant Science
Volume171
Issue number5
DOIs
Publication statusPublished - Nov 2006

Fingerprint

Abscisic Acid
Phosphoprotein Phosphatases
mitogen-activated protein kinase
abscisic acid
Phosphotransferases
Recovery
proteins
Arabidopsis
ethylene
phosphoprotein phosphatase
Osmotic Pressure
osmotic stress
Mitogens
Yeast
Protein Phosphatase 2C
stress response
phosphotransferases (kinases)
Yeasts
yeasts
mutants

Keywords

  • Abscisic acid
  • Cross-talk
  • Ethylene

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Antagonistic interaction between MAP kinase and protein phosphatase 2C in stress recovery. / Leung, Jeffrey; Orfanidi, Sofia; Chefdor, Françoise; Mészáros, T.; Bolte, Susanne; Mizoguchi, Tsuyoshi; Shinozaki, Kazuo; Giraudat, Jérôme; Bögre, Laszlo.

In: Plant Science, Vol. 171, No. 5, 11.2006, p. 596-606.

Research output: Contribution to journalArticle

Leung, J, Orfanidi, S, Chefdor, F, Mészáros, T, Bolte, S, Mizoguchi, T, Shinozaki, K, Giraudat, J & Bögre, L 2006, 'Antagonistic interaction between MAP kinase and protein phosphatase 2C in stress recovery', Plant Science, vol. 171, no. 5, pp. 596-606. https://doi.org/10.1016/j.plantsci.2006.06.009
Leung, Jeffrey ; Orfanidi, Sofia ; Chefdor, Françoise ; Mészáros, T. ; Bolte, Susanne ; Mizoguchi, Tsuyoshi ; Shinozaki, Kazuo ; Giraudat, Jérôme ; Bögre, Laszlo. / Antagonistic interaction between MAP kinase and protein phosphatase 2C in stress recovery. In: Plant Science. 2006 ; Vol. 171, No. 5. pp. 596-606.
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