Anomalous retention behaviour of some synthetic nucleosides on aluminium oxide layers

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Abstract

The retention of 21 nucleoside derivatives with various alkyl substituents at the 5′-position was determined on unimpregnated and paraffin oil-impregnated alumina layers using dichloroethane-methanol, water-methanol, water-ethanol and water-2-propanol eluent systems. The nucleosides showed anomalous retention behavior in each system: their retention first decreased with increasing concentration of the stronger component in the eluent, reached a maximum, and then increased with further increase in the concentration of the stronger component in the eluent. The data indicate a mixed (adsorption and reversed-phase) retentin mechanism independently of the impregnation of the alumina surface. A quadratic equation desribed well the dependence of the RM value of the concentration of the stronger component in the eluent; the parameters of the quadratic equation showed a high intercorrelation. The chain lenght of the alkyl substituent and the number of triple bonds in the alkyl chain governed the retention of nucleoside derivatives in each eluent system.

Original languageEnglish
Pages (from-to)425-438
Number of pages14
JournalJournal of Chromatography A
Volume543
Issue numberC
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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