Anomalous retention behaviour of peptides on porous graphitized carbon column

Veronika Nèmeth-Kiss, Esther Forgács, Tibor Cserháti

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Abstract

The retention behaviour of fourteen peptides was determined on a porous graphitized carbon (PGC) column, using acetonitrile-water mixtures as eluents. The majority of peptides showed irregular retention behaviour, their retention decreased with increasing concentration of acetonitrile in the lower concentration range, reached a minimum and increased again with increasing concentration of acetonitrile in the higher concentration range. Both quadratic and bilinear equations described well the irregular retention of peptides on the PGC column, however, the coefficients of correlation were slightly higher for the quadratic function. Calculations indicated that the impact of hydrophobicity, steric and electronic parameters on the retention of peptides on a PGC column is similar, that is a PGC column shows mixed retention mechanisms in which hydrophobic, steric, and electronic forces are equally involved.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalJournal of Chromatography A
Volume776
Issue number1
DOIs
Publication statusPublished - Jul 25 1997

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Keywords

  • Peptides
  • Porous graphite carbon
  • Principal component analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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