Annexin II modulates volume-activated chloride currents in vascular endothelial cells

Bernd Nilius, Volker Gerke, Jean Prenen, G. Szücs, Stephan Heinke, Klaus Weber, Guy Droogmans

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The membrane-associated, microfilament-binding protein annexin II is abundantly expressed in endothelial cells from calf pulmonary artery (CPAE cells). We have analyzed its role in the regulation of volume-activated chloride currents (I(Cl, vol)) by loading the cells via the patch pipette with a peptide comprising the N-terminal 14 residues of annexin II. This sequence harbors the binding site for the intracellular annexin II ligand, p11, and the peptide interferes with the annexin II-p11 complex formation. Loading of a CPAE cell with this peptide caused a gradual decrease in the amplitude of I(Cl, vol) during repetitive stimulations with a 28% hypotonic extracellular solution. This run down of the current was virtually absent in untreated cells and in cells that were loaded with a mutated 14-amino acid peptide, which has a single amino acid replacement known to result in a more than 1000 times reduced affinity for binding to p11. We conclude that annexin II-p11 complex formation is either directly or indirectly involved in the activation of I(Cl, vol) in endothelial cells.

Original languageEnglish
Pages (from-to)30631-30636
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number48
DOIs
Publication statusPublished - 1996

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Annexin A2
Endothelial cells
Chlorides
Endothelial Cells
Peptides
Hypotonic Solutions
Amino Acids
Microfilament Proteins
Ports and harbors
Pulmonary Artery
Carrier Proteins
Chemical activation
Binding Sites
Ligands
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Annexin II modulates volume-activated chloride currents in vascular endothelial cells. / Nilius, Bernd; Gerke, Volker; Prenen, Jean; Szücs, G.; Heinke, Stephan; Weber, Klaus; Droogmans, Guy.

In: Journal of Biological Chemistry, Vol. 271, No. 48, 1996, p. 30631-30636.

Research output: Contribution to journalArticle

Nilius, B, Gerke, V, Prenen, J, Szücs, G, Heinke, S, Weber, K & Droogmans, G 1996, 'Annexin II modulates volume-activated chloride currents in vascular endothelial cells', Journal of Biological Chemistry, vol. 271, no. 48, pp. 30631-30636. https://doi.org/10.1074/jbc.271.48.30631
Nilius, Bernd ; Gerke, Volker ; Prenen, Jean ; Szücs, G. ; Heinke, Stephan ; Weber, Klaus ; Droogmans, Guy. / Annexin II modulates volume-activated chloride currents in vascular endothelial cells. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 48. pp. 30631-30636.
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