Anisotropic Circular Dichroism of Light-Harvesting Complex II in Oriented Lipid Bilayers: Theory Meets Experiment

Parveen Akhtar, Dominik Lindorfer, Mónika Lingvay, Krzysztof Pawlak, Ottó Zsiros, Giuliano Siligardi, Tamás Jávorfi, Márta Dorogi, Bettina Ughy, G. Garab, Thomas Renger, P. Lambrev

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Anisotropic circular dichroism (ACD) spectroscopy of macroscopically aligned molecules reveals additional information about their excited states that is lost in the CD of randomly oriented solutions. ACD spectra of light-harvesting complex II (LHCII) - the main peripheral antenna of photosystem II in plants - in oriented lipid bilayers were recorded from the far-UV to the visible wavelength region. ACD spectra show a drastically enhanced magnitude and level of detail compared to the isotropic CD spectra, resolving a greater number of bands and weak optical transitions. Exciton calculations show that the spectral features in the chlorophyll Q y region are well-reproduced by an existing Hamiltonian for LHCII, providing further evidence for the identity of energy sinks at chlorophylls a603 and a610 in the stromal layer and chlorophylls a604 and a613 in the luminal layer. We propose ACD spectroscopy to be a valuable tool linking the three-dimensional structure and the photophysical properties of pigment-protein complexes.

Original languageEnglish
Pages (from-to)1090-1098
Number of pages9
JournalJournal of Physical Chemistry B
Volume123
Issue number5
DOIs
Publication statusPublished - Feb 7 2019

Fingerprint

Lipid bilayers
Lipid Bilayers
Chlorophyll
Circular Dichroism
Circular dichroism spectroscopy
dichroism
lipids
chlorophylls
Dichroism
Light
Hamiltonians
Spectrum Analysis
Optical transitions
Photosystem II Protein Complex
Experiments
Excited states
Pigments
Excitons
pigments
sinks

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Anisotropic Circular Dichroism of Light-Harvesting Complex II in Oriented Lipid Bilayers : Theory Meets Experiment. / Akhtar, Parveen; Lindorfer, Dominik; Lingvay, Mónika; Pawlak, Krzysztof; Zsiros, Ottó; Siligardi, Giuliano; Jávorfi, Tamás; Dorogi, Márta; Ughy, Bettina; Garab, G.; Renger, Thomas; Lambrev, P.

In: Journal of Physical Chemistry B, Vol. 123, No. 5, 07.02.2019, p. 1090-1098.

Research output: Contribution to journalArticle

Akhtar, P, Lindorfer, D, Lingvay, M, Pawlak, K, Zsiros, O, Siligardi, G, Jávorfi, T, Dorogi, M, Ughy, B, Garab, G, Renger, T & Lambrev, P 2019, 'Anisotropic Circular Dichroism of Light-Harvesting Complex II in Oriented Lipid Bilayers: Theory Meets Experiment', Journal of Physical Chemistry B, vol. 123, no. 5, pp. 1090-1098. https://doi.org/10.1021/acs.jpcb.8b12474
Akhtar, Parveen ; Lindorfer, Dominik ; Lingvay, Mónika ; Pawlak, Krzysztof ; Zsiros, Ottó ; Siligardi, Giuliano ; Jávorfi, Tamás ; Dorogi, Márta ; Ughy, Bettina ; Garab, G. ; Renger, Thomas ; Lambrev, P. / Anisotropic Circular Dichroism of Light-Harvesting Complex II in Oriented Lipid Bilayers : Theory Meets Experiment. In: Journal of Physical Chemistry B. 2019 ; Vol. 123, No. 5. pp. 1090-1098.
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