Angiotensin II and guanine nucleotides stimulate formation of inositol 1,4,5-trisphosphate and its metabolites in permeabilized adrenal glomerulosa cells

A. J. Baukal, T. Balla, L. Hunyady, W. Hausdorff, G. Guillemette, K. J. Catt

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Abstract

Angiotensin II (AII) interacts with specific receptors in the adrenal glomerulosa cell and stimulates the hydrolysis of plasma membrane phosphoinositides by phospholipase C, with production of inositol 1,4,5-trisphosphate (Ins-1,4,5-P3) and subsequent mobilization of intracellular Ca2+. In electrically permeabilized, [3H]inositol-labeled glomerulosa cells, AII stimulated Ins-1,4,5-P3 production within 15 s with half-maximal potency of 10-9 M. The nonhydrolyzable GTP analog, guanosine 5'-O-thiotriphosphate (GTPγS), stimulated Ins-1,4,5-P3 formation in a dose-dependent manner with half-maximal effect at 10-7 M. AII-activated Ins-1,4,5-P3 production was further increased by guanine nucleotides. The rate at which GTPγS-stimulated inositol polyphosphate production was consistently slower than that of AII. In adrenal membrane preparations, GTPγS-stimulated polyphosphoinositide hydrolysis was enhanced by Ca2+, with half-maximal activity at 300 nM free Ca2+. Ins-1,4,5-P3 formation was also increased by NaF, further indicating the involvement of a guanine nucleotide regulatory protein. In addition to Ins-1,4,5-P3 and its metabolites formed during degradation via the 4-monophosphate pathway, AII and GTPγS stimulated the formation of the phosphorylated metabolite inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in permeabilized cells. The absence of a significant rise in inositol 1-monophosphate indicated that phosphatidylinositol hydrolysis was not stimulated by AII or GTPγS. Pretreatment of glomerulosa cells with pertussis toxin for 12 h before permeabilization did not inhibit AII- or GTPγS-stimulated inositol polyphosphate formation. However, treatment with cholera toxin, forskolin, or 8-Br-cAMP for 12 h enhanced both basal and ligand-stimulated Ins-1,4,5-P3 production. These observations suggest that agonist binding to the AII receptor activates a polyphosphoinositide-specific phospholipase C in the adrenal glomerulosa cell, and that a distinctive guanine regulatory protein is involved in this mechanism.

Original languageEnglish
Pages (from-to)6087-6092
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number13
Publication statusPublished - Jan 1 1988

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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