Angiotensin II activates the human renin promoter in an in vitro model: The role of c-Jun-N-terminal kinase

Tamás Terebessy, András Masszi, A. Fintha, Attila Sebe, Tamás Huszár, L. Rosivall, I. Mucsi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background. Angiotensin II (Ang II) down-regulates renin expression in juxtaglomerular cells, however, recent experimental evidence obtained in transgenic mice suggested that Ang II may 'paradoxically' increase transcriptional activity of the proximal renin promoter. Methods. To dissect signalling mechanisms contributing to the up-regulation of the proximal renin promoter by Ang II, porcine proximal tubular cells stably expressing the rabbit AT1 receptor (LLC-PK/AT1) were transiently transfected with a luciferase reporter construct containing the 582 bp long piece of the human renin promoter. Activation of mitogen-activated protein kinases (MAPK) was detected by western blotting using phospho-MAPK-specific antibodies. Results. Ang II dose-dependently stimulated the transcriptional activity of the human renin promoter (10-7 M Ang II: 3.50±1.25-fold stimulation). In these cells Ang II activated both extracellular signal-regulated kinase (ERK) and the c-Jun-N-terminal kinase (JNK). Inhibition of the ERK cascade did not reduce the stimulation of the renin promoter by Ang II, however, two expression vectors designed to inhibit the JNK pathway, the dominant negative JNK and the Jun-kinase interacting peptide inhibited the fold stimulation induced by Ang II (2.75±0.69 vs 1.6±0.23 and 1.8± 0.34, respectively; P

Original languageEnglish
Pages (from-to)2184-2191
Number of pages8
JournalNephrology Dialysis Transplantation
Volume19
Issue number9
DOIs
Publication statusPublished - Sep 2004

Fingerprint

JNK Mitogen-Activated Protein Kinases
Renin
Angiotensin II
Phosphotransferases
Extracellular Signal-Regulated MAP Kinases
Mitogen-Activated Protein Kinases
In Vitro Techniques
Luciferases
Human Activities
Transgenic Mice
Up-Regulation
Swine
Down-Regulation
Western Blotting
Rabbits
Peptides
Antibodies

Keywords

  • Angiotensin II
  • Human renin promoter
  • Mitogen-activated protein kinases
  • Renal tubular cell
  • Transcriptional regulation
  • Tyrosine kinases

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Angiotensin II activates the human renin promoter in an in vitro model : The role of c-Jun-N-terminal kinase. / Terebessy, Tamás; Masszi, András; Fintha, A.; Sebe, Attila; Huszár, Tamás; Rosivall, L.; Mucsi, I.

In: Nephrology Dialysis Transplantation, Vol. 19, No. 9, 09.2004, p. 2184-2191.

Research output: Contribution to journalArticle

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