Angiotensin I conversion to angiotensin II stimulates cortical collecting duct sodium transport

Peter Komlosi, Amanda L. Fuson, Attila Fintha, János Peti-Peterdi, Laszlo Rosivall, David G. Warnock, Phillip Darwin Bell

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Angiotensin (Ang) II directly stimulates epithelial sodium channel activity in the rabbit cortical collecting duct. Because Ang I and converting enzyme analogues might be present in the distal nephron, this raises the possibility of intraluminal generation of Ang II. Conversion of Ang I to Ang II was monitored by Ang II-dependent changes in intracellular sodium concentration as a reflection of sodium transport across the apical membrane. This involved imaging-based fluorescence microscopy with sodium-binding benzofuran isophthalate in isolated, perfused, cortical collecting-duct segments from rabbit kidney. Principal and intercalated cells were differentiated by rhodamine-conjugated peanut lectin. Control principal cell intracellular sodium concentration, during perfusion with 25 mmol/L NaCl and zero sodium in the bath plus monensin (10-5 mol/L) averaged 5.8±0.14 mmol/L (n = 156). The increase in intracellular sodium concentration, when luminal NaCl was increased from 25 to 150 mmol/L, was elevated by 3.5-fold in the presence of intraluminal Ang I (10-6 mol/L). Also, the effects of Ang I on sodium transport were not significantly different from the effects of Ang II (10-9 mol/L). Ang I was used in micromolar concentrations to ensure that there was sufficient substrate available for conversion to Ang II. Inhibition of the angiotensin-converting enzyme with captopril reduced the stimulatory effect of Ang I. These results suggest that intraluminal conversion of Ang I to Ang II can occur in the cortical collecting duct, resulting in enhanced apical sodium entry.

Original languageEnglish
Pages (from-to)195-199
Number of pages5
JournalHypertension
Volume42
Issue number2
DOIs
Publication statusPublished - Aug 1 2003

Keywords

  • Angiotensin-converting enzyme
  • Imaging
  • Kallikrein
  • Kidney
  • Rabbits
  • Renin-angiotensin system

ASJC Scopus subject areas

  • Internal Medicine

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