ANF (Arg 101 - Tyr 126) is the peptide secreted by rat atrial cardiocytes in culture

J. Gutkowska, C. Lazure, K. Racz, G. Thibault, R. Garcia, N. G. Seidah, M. Chrétien, J. Genest, M. Cantin

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6 Citations (Scopus)

Abstract

The atrial natriuretic factor (ANF) secreted from rat cardiocytes in culture was purified and characterized. The purification procedure involves extraction of ANF by activated Vycor glass, followed by HPLC on C18 μ Bondapak and Vydac columns. The detection of ANF in column eluates was performed by a simple and sensitive radioimmunoassay. The amino acid composition and N-terminal amino acid sequencing appeared to be identical to the Arg 101 - Tyr 126 peptide. The isolated ANF showed biological activity, inhibiting basal and ACTH-stimulating aldosterone secretion from rat zona glomerulosa cells with the same potency as the synthetic peptide.

Original languageEnglish
Pages (from-to)1217-1225
Number of pages9
JournalBiochemical and biophysical research communications
Volume130
Issue number3
DOIs
Publication statusPublished - Aug 15 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Gutkowska, J., Lazure, C., Racz, K., Thibault, G., Garcia, R., Seidah, N. G., Chrétien, M., Genest, J., & Cantin, M. (1985). ANF (Arg 101 - Tyr 126) is the peptide secreted by rat atrial cardiocytes in culture. Biochemical and biophysical research communications, 130(3), 1217-1225. https://doi.org/10.1016/0006-291X(85)91744-9