Andrology: Results of 55 intracytoplasmic sperm injection cycles in the treatment of male-immunological infertility

Z. P. Nagy, G. Verheyen, J. Liu, H. Joris, C. Janssenswillen, A. Wisanto, P. Devroey, A. C. Van Steirteghem

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Antisperm antibodies present in the semen can be a primary cause of infertility. If the proportion of spermatozoa carrying antisperm antibodies is very high, then usually a poor result ensues in standard in-vitro fertilization. We therefore employed intracytoplasmic sperm injection (ICSI) in 55 cycles (37 patients) where the proportion of antisperm antibody-bound spermatozoa was 80% or higher, as determined by the mixed antiglobulin reaction (MAR) test. The type and location of antisperm antibodies were determined by the immunobead test in 30 of the 37 patients. The mean normal fertilization rate was 75.7% in these 55 cycles, which was significantly higher than the fertilization rate in another 1767 ICSI cycles (69.2%) performed over the same period and where MAR-negative semen (the level of antisperm antibodies was <80%) was used for microinjection. Embryonic development was comparable, but a higher proportion of poor-quality embryos was obtained with MAR-positive than with MAR-negative semen samples. Out of the 55 patients, 53 had embryos replaced (96.4%) and a fetal sac was detected by ultrasonography in 14 patients (26.4%). The data indicate that fertilization, embryo development and pregnancy rates after ICSI are not influenced significantly by the proportion of antisperm antibody-bound spermatozoa, nor by the dominant type of antibodies present, nor by the location of the antisperm antibody on the spermatozoa. The conclusion of this study is that ICSI should be the primary choice for patients who have high numbers of antisperm antibodies present in their semen.

Original languageEnglish
Pages (from-to)1775-1780
Number of pages6
JournalHuman Reproduction
Issue number7
Publication statusPublished - Jul 1995



  • Antisperm antibody
  • Fertilization
  • ICSI
  • Immunological infertility
  • Microinjection

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Nagy, Z. P., Verheyen, G., Liu, J., Joris, H., Janssenswillen, C., Wisanto, A., Devroey, P., & Van Steirteghem, A. C. (1995). Andrology: Results of 55 intracytoplasmic sperm injection cycles in the treatment of male-immunological infertility. Human Reproduction, 10(7), 1775-1780.