Andrology

Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration

Sherman J. Silber, P. Nagy, Jiaen Liu, Hugo Godoy, Paul Devroey, André C. Van Steirteghem

Research output: Contribution to journalArticle

284 Citations (Scopus)

Abstract

Intracytoplasmic sperm injection (ICSI) has been successful in cases of extreme oligoasthenozoospermia in achieving pregnancies via in-vitro fertilization (IVF) with the lowest imaginable sperm counts. In azoospermia caused by congenital bilateral absence of the vas deferens (CBAVD), it has been shown that epididymal spermatozoa can be retrieved in large numbers, but fertilization rates using conventional IVF are low. Furthermore, no fertilization has ever been possible using testicular spermatozoa with conventional IVF. In the most extreme case of absence of the epididymis, spermatozoa can only be retrieved from macerated testicular biopsy specimens. In such cases, all that can be seen are free-floating Sertoli cells with many spermatids attached, and only occasional spermatozoa per high power field which have only the barest, occasional, slightly twitching motion. The objective of the present study was to determine whether ICSI could achieve better results than conventional IVF with microsurgical aspiration of spermatozoa (MESA). ICSI (using epididymal or testicular spermatozoa) from men with CBAVD or irreparable obstructive azoospermia, achieved good fertilization and normal embryos in 82% of cases, compared to 19% with conventional IVF. There was an overall fertilization rate of 45%, with 85% progressing to normally cleaving embryos using ICSI, compared to 6.9% using conventional IVF. The pregnancy rate with ICSI/MESA was 47% per stimulated cycle (normal delivery rate was 30%), compared to 4.5% with conventional IVF. These results were achieved in patients who had consistently failed to fertilize in previous cycles with MESA and conventional IVF. We conclude that although complex mechanisms (facilitated by epididymal passage) may be required by spermatozoa for conventional fertilization of human oocytes (whether in vivo or in vitro), no such mechanisms are required for fertilization after direct microinjection. Because of the consistently good results using epididymal spermatozoa with ICSI in comparison to conventional IVF, and also the good results in extreme cases requiring testicular tissue spermatozoa, ICSI may be man dated for all future MESA patients with CBAVD, or with irreparable obstructive azoospermia.

Original languageEnglish
Pages (from-to)1705-1709
Number of pages5
JournalHuman Reproduction
Volume9
Issue number9
Publication statusPublished - Sep 1994

Fingerprint

Sperm Retrieval
Intracytoplasmic Sperm Injection
Andrology
Spermatozoa
In Vitro Fertilization
Intracytoplasmic Sperm Injections
Biopsy
Fertilization in Vitro
Fertilization
Tissue
Azoospermia
Extremes
Pregnancy
Embryo
Embryonic Structures
Human Oocytes
Oligospermia
Sperm
Aspiration
In vitro fertilization

Keywords

  • Epididymal
  • Fertilization
  • Injection
  • Intracytoplasmic
  • Sperm

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Rehabilitation
  • Physiology
  • Developmental Biology

Cite this

Andrology : Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. / Silber, Sherman J.; Nagy, P.; Liu, Jiaen; Godoy, Hugo; Devroey, Paul; Van Steirteghem, André C.

In: Human Reproduction, Vol. 9, No. 9, 09.1994, p. 1705-1709.

Research output: Contribution to journalArticle

Silber, Sherman J. ; Nagy, P. ; Liu, Jiaen ; Godoy, Hugo ; Devroey, Paul ; Van Steirteghem, André C. / Andrology : Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. In: Human Reproduction. 1994 ; Vol. 9, No. 9. pp. 1705-1709.
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abstract = "Intracytoplasmic sperm injection (ICSI) has been successful in cases of extreme oligoasthenozoospermia in achieving pregnancies via in-vitro fertilization (IVF) with the lowest imaginable sperm counts. In azoospermia caused by congenital bilateral absence of the vas deferens (CBAVD), it has been shown that epididymal spermatozoa can be retrieved in large numbers, but fertilization rates using conventional IVF are low. Furthermore, no fertilization has ever been possible using testicular spermatozoa with conventional IVF. In the most extreme case of absence of the epididymis, spermatozoa can only be retrieved from macerated testicular biopsy specimens. In such cases, all that can be seen are free-floating Sertoli cells with many spermatids attached, and only occasional spermatozoa per high power field which have only the barest, occasional, slightly twitching motion. The objective of the present study was to determine whether ICSI could achieve better results than conventional IVF with microsurgical aspiration of spermatozoa (MESA). ICSI (using epididymal or testicular spermatozoa) from men with CBAVD or irreparable obstructive azoospermia, achieved good fertilization and normal embryos in 82{\%} of cases, compared to 19{\%} with conventional IVF. There was an overall fertilization rate of 45{\%}, with 85{\%} progressing to normally cleaving embryos using ICSI, compared to 6.9{\%} using conventional IVF. The pregnancy rate with ICSI/MESA was 47{\%} per stimulated cycle (normal delivery rate was 30{\%}), compared to 4.5{\%} with conventional IVF. These results were achieved in patients who had consistently failed to fertilize in previous cycles with MESA and conventional IVF. We conclude that although complex mechanisms (facilitated by epididymal passage) may be required by spermatozoa for conventional fertilization of human oocytes (whether in vivo or in vitro), no such mechanisms are required for fertilization after direct microinjection. Because of the consistently good results using epididymal spermatozoa with ICSI in comparison to conventional IVF, and also the good results in extreme cases requiring testicular tissue spermatozoa, ICSI may be man dated for all future MESA patients with CBAVD, or with irreparable obstructive azoospermia.",
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