Androgens negatively regulate myostatin expression in an androgen-dependent skeletal muscle

Luca Mendler, Zsuzsanna Baka, Andrea Kovács-Simon, L. Dux

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Myostatin is an important negative regulator of skeletal muscle growth, while androgens are strong positive effectors. In order to investigate the possible interaction between myostatin and androgen pathways, we followed myostatin expression in the androgen-dependent levator ani (LA) muscle of the rat as a function of androgen status. By testosterone deprivation (castration), we induced LA growth arrest in young male rats, whilst atrophy in adult ones, however, both processes could be reversed by testosterone supplementation. After castration, a significant up-regulation of active myostatin protein (and its propeptide) was found, whereas the subsequent testosterone treatment reduced myostatin protein levels to normal values in both young and adult rats. Similarly, a testosterone-induced suppression of myostatin mRNA levels was observed in castrated adult but not in young animals. Altogether, androgens seem to have strong negative impact on myostatin expression, which might be a key factor in the weight regulation of LA muscle.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume361
Issue number1
DOIs
Publication statusPublished - Sep 14 2007

Fingerprint

Myostatin
Androgens
Muscle
Skeletal Muscle
Testosterone
Anal Canal
Rats
Castration
Muscles
Growth
Atrophy
Young Adult
Reference Values
Animals
Proteins
Up-Regulation
Weights and Measures
Messenger RNA

Keywords

  • Androgen
  • Castration
  • Levator ani
  • Myostatin
  • Rat
  • Testosterone supplementation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Androgens negatively regulate myostatin expression in an androgen-dependent skeletal muscle. / Mendler, Luca; Baka, Zsuzsanna; Kovács-Simon, Andrea; Dux, L.

In: Biochemical and Biophysical Research Communications, Vol. 361, No. 1, 14.09.2007, p. 237-242.

Research output: Contribution to journalArticle

Mendler, Luca ; Baka, Zsuzsanna ; Kovács-Simon, Andrea ; Dux, L. / Androgens negatively regulate myostatin expression in an androgen-dependent skeletal muscle. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 361, No. 1. pp. 237-242.
@article{fddb060708ee45958a5d2f2ba213659b,
title = "Androgens negatively regulate myostatin expression in an androgen-dependent skeletal muscle",
abstract = "Myostatin is an important negative regulator of skeletal muscle growth, while androgens are strong positive effectors. In order to investigate the possible interaction between myostatin and androgen pathways, we followed myostatin expression in the androgen-dependent levator ani (LA) muscle of the rat as a function of androgen status. By testosterone deprivation (castration), we induced LA growth arrest in young male rats, whilst atrophy in adult ones, however, both processes could be reversed by testosterone supplementation. After castration, a significant up-regulation of active myostatin protein (and its propeptide) was found, whereas the subsequent testosterone treatment reduced myostatin protein levels to normal values in both young and adult rats. Similarly, a testosterone-induced suppression of myostatin mRNA levels was observed in castrated adult but not in young animals. Altogether, androgens seem to have strong negative impact on myostatin expression, which might be a key factor in the weight regulation of LA muscle.",
keywords = "Androgen, Castration, Levator ani, Myostatin, Rat, Testosterone supplementation",
author = "Luca Mendler and Zsuzsanna Baka and Andrea Kov{\'a}cs-Simon and L. Dux",
year = "2007",
month = "9",
day = "14",
doi = "10.1016/j.bbrc.2007.07.023",
language = "English",
volume = "361",
pages = "237--242",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Androgens negatively regulate myostatin expression in an androgen-dependent skeletal muscle

AU - Mendler, Luca

AU - Baka, Zsuzsanna

AU - Kovács-Simon, Andrea

AU - Dux, L.

PY - 2007/9/14

Y1 - 2007/9/14

N2 - Myostatin is an important negative regulator of skeletal muscle growth, while androgens are strong positive effectors. In order to investigate the possible interaction between myostatin and androgen pathways, we followed myostatin expression in the androgen-dependent levator ani (LA) muscle of the rat as a function of androgen status. By testosterone deprivation (castration), we induced LA growth arrest in young male rats, whilst atrophy in adult ones, however, both processes could be reversed by testosterone supplementation. After castration, a significant up-regulation of active myostatin protein (and its propeptide) was found, whereas the subsequent testosterone treatment reduced myostatin protein levels to normal values in both young and adult rats. Similarly, a testosterone-induced suppression of myostatin mRNA levels was observed in castrated adult but not in young animals. Altogether, androgens seem to have strong negative impact on myostatin expression, which might be a key factor in the weight regulation of LA muscle.

AB - Myostatin is an important negative regulator of skeletal muscle growth, while androgens are strong positive effectors. In order to investigate the possible interaction between myostatin and androgen pathways, we followed myostatin expression in the androgen-dependent levator ani (LA) muscle of the rat as a function of androgen status. By testosterone deprivation (castration), we induced LA growth arrest in young male rats, whilst atrophy in adult ones, however, both processes could be reversed by testosterone supplementation. After castration, a significant up-regulation of active myostatin protein (and its propeptide) was found, whereas the subsequent testosterone treatment reduced myostatin protein levels to normal values in both young and adult rats. Similarly, a testosterone-induced suppression of myostatin mRNA levels was observed in castrated adult but not in young animals. Altogether, androgens seem to have strong negative impact on myostatin expression, which might be a key factor in the weight regulation of LA muscle.

KW - Androgen

KW - Castration

KW - Levator ani

KW - Myostatin

KW - Rat

KW - Testosterone supplementation

UR - http://www.scopus.com/inward/record.url?scp=34547093645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547093645&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.07.023

DO - 10.1016/j.bbrc.2007.07.023

M3 - Article

C2 - 17658471

AN - SCOPUS:34547093645

VL - 361

SP - 237

EP - 242

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -