Anatomical and physiological evidence for involvement of tuberoinfundibular peptide of 39 residues in nociception

A. Dobolyi, Hiroshi Ueda, Hitoshi Uchida, M. Palkóvits, Ted B. Usdin

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The parathyroid hormone 2 (PTH2) receptor's anatomical distribution suggests that, among other functions, it may be involved in modulation of nociception. We localized PTH2 receptor protein to spinal cord lamina II and showed that it is synthesized by sub-populations of primary sensory neurons and intrinsic spinal cord dorsal horn neurons. Tuberoinfundibular peptide of 39 residues (TIP39) selectively activates the PTH2 receptor. Intraplantar microinjection of TIP39 caused a paw-withdrawal response and intrathecal injection caused scratching, biting, and licking, a nocifensive response. Intrathecal administration of a TIP39 antibody decreased sensitivity in tail-flick and paw-pressure assays. Intrathecal administration of TIP39 potentiated responses in these assays. We determined the sequence of TIP39's precursor and found that mRNA encoding TIP39 and TIP39-like immunoreactivity is concentrated in two brainstem areas, the subparafascicular area and the caudal paralemniscal nucleus. Cells in these areas project to the superficial dorsal horn of the spinal cord. Our data suggest that TIP39 released from supraspinal fibers potentiates aspects of nociception within the spinal cord.

Original languageEnglish
Pages (from-to)1651-1656
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number3
DOIs
Publication statusPublished - Feb 5 2002

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Nociception
Receptor, Parathyroid Hormone, Type 2
Spinal Cord
Substantia Gelatinosa
Posterior Horn Cells
Spinal Injections
RNA Precursors
Microinjections
Sensory Receptor Cells
tuberoinfundibular peptide 39
Brain Stem
Pressure
Antibodies
Population

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Anatomical and physiological evidence for involvement of tuberoinfundibular peptide of 39 residues in nociception. / Dobolyi, A.; Ueda, Hiroshi; Uchida, Hitoshi; Palkóvits, M.; Usdin, Ted B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 3, 05.02.2002, p. 1651-1656.

Research output: Contribution to journalArticle

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