Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks

Ryanne I. Jaarsma, Hein Sprong, Katsuhisa Takumi, Maria Kazimirova, Cornelia Silaghi, Atle Mysterud, Ivo Rudolf, Relja Beck, G. Földvári, Laura Tomassone, Margit Groenevelt, Reinard R. Everts, Jolianne M. Rijks, Frauke Ecke, Birger Hörnfeldt, David Modrý, Karolina Majerová, Jan Votýpka, Agustín Estrada-Peña

Research output: Contribution to journalArticle

Abstract

Background: Anaplasma phagocytophilum is currently regarded as a single species. However, molecular studies indicate that it can be subdivided into ecotypes, each with distinct but overlapping transmission cycle. Here, we evaluate the interactions between and within clusters of haplotypes of the bacterium isolated from vertebrates and ticks, using phylogenetic and network-based methods. Methods: The presence of A. phagocytophilum DNA was determined in ticks and vertebrate tissue samples. A fragment of the groEl gene was amplified and sequenced from qPCR-positive lysates. Additional groEl sequences from ticks and vertebrate reservoirs were obtained from GenBank and through literature searches, resulting in a dataset consisting of 1623 A. phagocytophilum field isolates. Phylogenetic analyses were used to infer clusters of haplotypes and to assess phylogenetic clustering of A. phagocytophilum in vertebrates or ticks. Network-based methods were used to resolve host-vector interactions and their relative importance in the segregating communities of haplotypes. Results: Phylogenetic analyses resulted in 199 haplotypes within eight network-derived clusters, which were allocated to four ecotypes. The interactions of haplotypes between ticks, vertebrates and geographical origin, were visualized and quantified from networks. A high number of haplotypes were recorded in the tick Ixodes ricinus. Communities of A. phagocytophilum recorded from Korea, Japan, Far Eastern Russia, as well as those associated with rodents had no links with the larger set of isolates associated with I. ricinus, suggesting different evolutionary pressures. Rodents appeared to have a range of haplotypes associated with either Ixodes trianguliceps or Ixodes persulcatus and Ixodes pavlovskyi. Haplotypes found in rodents in Russia had low similarities with those recorded in rodents in other regions and shaped separate communities. Conclusions: The groEl gene fragment of A. phagocytophilum provides information about spatial segregation and associations of haplotypes to particular vector-host interactions. Further research is needed to understand the circulation of this bacterium in the gap between Europe and Asia before the overview of the speciation features of this bacterium is complete. Environmental traits may also play a role in the evolution of A. phagocytophilum in ecotypes through yet unknown relationships.

Original languageEnglish
Article number328
JournalParasites and Vectors
Volume12
Issue number1
DOIs
Publication statusPublished - Jun 28 2019

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Anaplasma phagocytophilum
Ticks
Haplotypes
Vertebrates
Ixodes
Ecotype
Rodentia
Russia
Bacteria
Nucleic Acid Databases
Korea
Genes
Cluster Analysis
Japan

Keywords

  • Anaplasma phagocytophilum
  • Ixodidae
  • Molecular epidemiology
  • Network analysis
  • Ticks
  • Transmission dynamics

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Jaarsma, R. I., Sprong, H., Takumi, K., Kazimirova, M., Silaghi, C., Mysterud, A., ... Estrada-Peña, A. (2019). Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks. Parasites and Vectors, 12(1), [328]. https://doi.org/10.1186/s13071-019-3583-8

Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks. / Jaarsma, Ryanne I.; Sprong, Hein; Takumi, Katsuhisa; Kazimirova, Maria; Silaghi, Cornelia; Mysterud, Atle; Rudolf, Ivo; Beck, Relja; Földvári, G.; Tomassone, Laura; Groenevelt, Margit; Everts, Reinard R.; Rijks, Jolianne M.; Ecke, Frauke; Hörnfeldt, Birger; Modrý, David; Majerová, Karolina; Votýpka, Jan; Estrada-Peña, Agustín.

In: Parasites and Vectors, Vol. 12, No. 1, 328, 28.06.2019.

Research output: Contribution to journalArticle

Jaarsma, RI, Sprong, H, Takumi, K, Kazimirova, M, Silaghi, C, Mysterud, A, Rudolf, I, Beck, R, Földvári, G, Tomassone, L, Groenevelt, M, Everts, RR, Rijks, JM, Ecke, F, Hörnfeldt, B, Modrý, D, Majerová, K, Votýpka, J & Estrada-Peña, A 2019, 'Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks', Parasites and Vectors, vol. 12, no. 1, 328. https://doi.org/10.1186/s13071-019-3583-8
Jaarsma RI, Sprong H, Takumi K, Kazimirova M, Silaghi C, Mysterud A et al. Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks. Parasites and Vectors. 2019 Jun 28;12(1). 328. https://doi.org/10.1186/s13071-019-3583-8
Jaarsma, Ryanne I. ; Sprong, Hein ; Takumi, Katsuhisa ; Kazimirova, Maria ; Silaghi, Cornelia ; Mysterud, Atle ; Rudolf, Ivo ; Beck, Relja ; Földvári, G. ; Tomassone, Laura ; Groenevelt, Margit ; Everts, Reinard R. ; Rijks, Jolianne M. ; Ecke, Frauke ; Hörnfeldt, Birger ; Modrý, David ; Majerová, Karolina ; Votýpka, Jan ; Estrada-Peña, Agustín. / Anaplasma phagocytophilum evolves in geographical and biotic niches of vertebrates and ticks. In: Parasites and Vectors. 2019 ; Vol. 12, No. 1.
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AU - Sprong, Hein

AU - Takumi, Katsuhisa

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AU - Silaghi, Cornelia

AU - Mysterud, Atle

AU - Rudolf, Ivo

AU - Beck, Relja

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AU - Groenevelt, Margit

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AU - Ecke, Frauke

AU - Hörnfeldt, Birger

AU - Modrý, David

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