Analytical separation of reduced and oxidized forms of glutathione from amino acid mixtures by overpressured thin-layer chromatography

Magdolna Ábraham, Béla Polyák, Béla Szajáni, László Boross

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new method has been developed for the separation of reduced and oxidized forms of glutathione from amino acid mixtures. The samples were spotted on Kieselgel plates and developed in phenol-water = 7:3 containing SDS. The separation was performed in pressurized ultramicro chamber. The running time was shorter than that in a normal chamber, decreasing the possibility of diffusion. In addition to the two forms of glutathione, nine different kinds of amino acids could be separated. The method affords a possibility for rapid analysis of two forms of glutathione both in the clinical and industrial practice.

Original languageEnglish
Pages (from-to)2635-2645
Number of pages11
JournalJournal of Liquid Chromatography
Volume6
Issue number14
DOIs
Publication statusPublished - Dec 1 1983

ASJC Scopus subject areas

  • Molecular Medicine

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