Analytical and structural studies for the investigation of oxidative stress in guanine oligonucleotides

Györgyi Ferenc, Zoltán Váradi, Zoltán Kupihár, Gábor Paragi, Lajos Kovács

Research output: Contribution to journalReview article

Abstract

DNA damage plays a decisive role in epigenetic effects. The detection and analysis of DNA damages, like the most common change of guanine (G) to 8-oxo-7,8-dihydroguanine (OG), is a key factor in cancer research. It is especially true for G quadruplex structure (GQ), which is one of the best-known examples of a non-canonical DNA arrangement. In the present work, we provided an overview on analytical methods in connection with the detection of OG in oligonucleotides with GQ-forming capacity. Focusing on the last five years, novel electrochemical tools, like dedicated electrodes, were overviewed, as well as different optical methods (fluorometric assays, resonance light scattering or UV radiation) along with hyphenated detection and structural analysis methods (CD, NMR, melting temperature analysis and nanopore detection) were also applied for OG detection. Additionally, GQ-related computational simulations were also summarized. All these results emphasize that OG detection and the analysis of the effect of its presence in higher ordered structures like GQ is still a state-of-the-art research line with continuously increasing interest.

Original languageEnglish
Article number4981
Pages (from-to)1-27
Number of pages27
JournalInternational journal of molecular sciences
Volume21
Issue number14
DOIs
Publication statusPublished - Jul 2 2020

Keywords

  • 8-oxo-7,8-dihydro-2-deoxyguanosine
  • Analytical methods
  • Computational studies
  • Epigenetic
  • Guanine
  • Oxidative stress
  • Quadruplex
  • Reactive nitrogen species
  • Reactive oxygen species
  • Structural investigations

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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