Analytical and micropreparative ultrahigh resolution of oligonucleotides by polyacrylamide gel high-performance capillary electrophoresis

A. Guttman, A. S. Cohen, D. N. Heiger, B. L. Karger

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

In continuation of previous work, the separations of polydeoxyoligonucleotides on polyacrylamide gel capillary columns are presented. The use of gel compositions with relatively low monomer content permits columns of very high plate numbers to be obtained. With a 160-mer, plate counts of 30 × 106 per meter are shown. Columns with high precision in relative migration time from run to run, day to day, and batch to batch are presented. Collection of purified fractions from high-resolution electropherograms is also shown to be feasible using field programming techniques. To accomplish this, separation is allowed to occur under high field for resolution and speed, followed by collection under low field where the velocity of the band is purposely decreased in order to widen the time-based width of the band.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalAnalytical Chemistry
Volume62
Issue number2
Publication statusPublished - Jan 15 1990

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Capillary electrophoresis
Oligonucleotides
Monomers
Gels
Chemical analysis
polyacrylamide gels

ASJC Scopus subject areas

  • Analytical Chemistry

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Analytical and micropreparative ultrahigh resolution of oligonucleotides by polyacrylamide gel high-performance capillary electrophoresis. / Guttman, A.; Cohen, A. S.; Heiger, D. N.; Karger, B. L.

In: Analytical Chemistry, Vol. 62, No. 2, 15.01.1990, p. 137-141.

Research output: Contribution to journalArticle

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