Analytical and micropreparative capillary gel electrophoresis of DNA fragments

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Abstract

In this study, automated capillary gel electrophoresis was applied for high-resolution analytical and high-yield micropreparative separation of DNA fragments using replaceable polymer networks and multispectral detection modes. The excellent separation power of high-performance CGE allowed high-resolution analysis of very similar-size DNA molecules and precise collection of any required fragments for further downstream processing. Nanomolar quantities of cDNA amplicons were collected, and subsequent cloning experiments were successfully accomplished.

Original languageEnglish
Pages28-31
Number of pages4
Volume32
No.14
Specialist publicationAmerican Laboratory
Publication statusPublished - Jul 1 2000

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ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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