Analysis of viral epigenotypes using bisulfite sequencing: A detailed protocol for the crucial bisulfite modification and PCR amplification steps

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Characterization of viral DNA methylation patterns is essential to understand its function in viral pathogenesis. Bisulfite modification, followed by polymerase chain reaction (PCR) and sequencing is the most effective method for the high resolution methylation mapping of viral genomes. Since the bisulfite modification and PCR steps are the most critical ones, an optimized protocol for these two steps is presented, with special attention to potential pitfalls.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages207-213
Number of pages7
Volume1532
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1532
ISSN (Print)10643745

Keywords

  • Bisulfite modification
  • DNA methylation
  • Polymerase chain reaction
  • Primer design
  • Sequencing
  • Virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Analysis of viral epigenotypes using bisulfite sequencing: A detailed protocol for the crucial bisulfite modification and PCR amplification steps'. Together they form a unique fingerprint.

  • Cite this