Analysis of transforming gene regions of human papillomavirus type 16 in normal cervical smears

J. Czeglédy, I. Batár, M. Evander, L. Gergely, G. Wadell

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Exfoliated cells from the uterine cervix of 102 Hungarian women with no cytological abnormality were screened using the polymerase chain reaction (PCR) for human papillomavirus (HPV) type 16 infection. Twenty-nine patients with histologically confirmed cervical intraepithelial neoplasia (CIN) served as reference cases. PCR was performed with 2 different HPV 16 specific oligonucleotide primer pairs flanking a 300 and a 200 base-pair fragment from the early 6 (E6) and early 7 (E7) genes, position 215-514 and 605-805. The specimens exhibited the same proportions of type 16 sequences specific for the tested regions. 8.8% (9/102) of normal samples showed amplification for HPV type 16 E6 and E7 regions, while 48.3% (14/29) of CIN biopsies were positive for the same gene sequences.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalArchives of Gynecology and Obstetrics
Volume249
Issue number4
DOIs
Publication statusPublished - Dec 1991

Fingerprint

Vaginal Smears
Human papillomavirus 16
Oncogenes
Cervical Intraepithelial Neoplasia
Polymerase Chain Reaction
DNA Primers
Gene Order
Papillomavirus Infections
Cervix Uteri
Base Pairing
Biopsy
Genes

Keywords

  • Cervical HPV 16 infection
  • Cervical intraepithelial neoplasias
  • Normal cervical smears
  • PCR

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Analysis of transforming gene regions of human papillomavirus type 16 in normal cervical smears. / Czeglédy, J.; Batár, I.; Evander, M.; Gergely, L.; Wadell, G.

In: Archives of Gynecology and Obstetrics, Vol. 249, No. 4, 12.1991, p. 185-189.

Research output: Contribution to journalArticle

Czeglédy, J. ; Batár, I. ; Evander, M. ; Gergely, L. ; Wadell, G. / Analysis of transforming gene regions of human papillomavirus type 16 in normal cervical smears. In: Archives of Gynecology and Obstetrics. 1991 ; Vol. 249, No. 4. pp. 185-189.
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