Analysis of the relationship between growth, cephalosporin C production, and fragmentation in Acremonium chrysogenum

E. Sándor, A. Szentirmai, G. C. Paul, C. R. Thomas, I. Pócsi, L. Karaffa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mycelial fragmentation in submerged cultures of the cephalosporin C (CPC) producing fungus Acremonium chrysogenum was characterized by image analysis. In both fed-batch and chemostat cultures, the proportion of mycelial clumps seemed to be the most sensitive morphological indicator of fragmentation. In a fed-batch fermentation culture, this declined from roughly 60% at inoculation to less than 10% after 43 h. Subsequent additions of glucose resulted in a sharp increase back to near the initial value, an increase that reversed itself a few hours after glucose exhaustion. Meanwhile CPC production continued to decline steadily. On the other hand, the addition of soybean oil enhanced CPC production, but had no significant effect on the morphology. Although it may sometimes appear that morphology and productivity are related in batch or fed-batch cultures, this study suggests that this is because both respond simultaneously to more fundamental physiological changes, dependent on the availability of carbon. In circumstances, such as supplementary carbon source addition, the relationship is lost. Chemostat cultures supported this belief, as CPC-production rates were hardly affected by the specific growth rate, but the morphology showed significant differences, i.e., lower dilution rates resulted in a lower proportion of clumps and in smaller clumps.

Original languageEnglish
Pages (from-to)801-806
Number of pages6
JournalCanadian Journal of Microbiology
Volume47
Issue number9
DOIs
Publication statusPublished - 2001

Fingerprint

Acremonium
Batch Cell Culture Techniques
Chemostats
Growth
Carbon
Glucose
Soybean Oil
Fungi
Fermentation
Image analysis
Dilution
Productivity
Availability
cephalosporin C

Keywords

  • Acremonium chrysogenum
  • Cephalosporin C
  • Fragmentation
  • Image analysis
  • Morphology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology

Cite this

@article{47ad53235e6f4f998613723a32b06b96,
title = "Analysis of the relationship between growth, cephalosporin C production, and fragmentation in Acremonium chrysogenum",
abstract = "Mycelial fragmentation in submerged cultures of the cephalosporin C (CPC) producing fungus Acremonium chrysogenum was characterized by image analysis. In both fed-batch and chemostat cultures, the proportion of mycelial clumps seemed to be the most sensitive morphological indicator of fragmentation. In a fed-batch fermentation culture, this declined from roughly 60{\%} at inoculation to less than 10{\%} after 43 h. Subsequent additions of glucose resulted in a sharp increase back to near the initial value, an increase that reversed itself a few hours after glucose exhaustion. Meanwhile CPC production continued to decline steadily. On the other hand, the addition of soybean oil enhanced CPC production, but had no significant effect on the morphology. Although it may sometimes appear that morphology and productivity are related in batch or fed-batch cultures, this study suggests that this is because both respond simultaneously to more fundamental physiological changes, dependent on the availability of carbon. In circumstances, such as supplementary carbon source addition, the relationship is lost. Chemostat cultures supported this belief, as CPC-production rates were hardly affected by the specific growth rate, but the morphology showed significant differences, i.e., lower dilution rates resulted in a lower proportion of clumps and in smaller clumps.",
keywords = "Acremonium chrysogenum, Cephalosporin C, Fragmentation, Image analysis, Morphology",
author = "E. S{\'a}ndor and A. Szentirmai and Paul, {G. C.} and Thomas, {C. R.} and I. P{\'o}csi and L. Karaffa",
year = "2001",
doi = "10.1139/cjm-47-9-801",
language = "English",
volume = "47",
pages = "801--806",
journal = "Canadian Journal of Microbiology",
issn = "0008-4166",
publisher = "National Research Council of Canada",
number = "9",

}

TY - JOUR

T1 - Analysis of the relationship between growth, cephalosporin C production, and fragmentation in Acremonium chrysogenum

AU - Sándor, E.

AU - Szentirmai, A.

AU - Paul, G. C.

AU - Thomas, C. R.

AU - Pócsi, I.

AU - Karaffa, L.

PY - 2001

Y1 - 2001

N2 - Mycelial fragmentation in submerged cultures of the cephalosporin C (CPC) producing fungus Acremonium chrysogenum was characterized by image analysis. In both fed-batch and chemostat cultures, the proportion of mycelial clumps seemed to be the most sensitive morphological indicator of fragmentation. In a fed-batch fermentation culture, this declined from roughly 60% at inoculation to less than 10% after 43 h. Subsequent additions of glucose resulted in a sharp increase back to near the initial value, an increase that reversed itself a few hours after glucose exhaustion. Meanwhile CPC production continued to decline steadily. On the other hand, the addition of soybean oil enhanced CPC production, but had no significant effect on the morphology. Although it may sometimes appear that morphology and productivity are related in batch or fed-batch cultures, this study suggests that this is because both respond simultaneously to more fundamental physiological changes, dependent on the availability of carbon. In circumstances, such as supplementary carbon source addition, the relationship is lost. Chemostat cultures supported this belief, as CPC-production rates were hardly affected by the specific growth rate, but the morphology showed significant differences, i.e., lower dilution rates resulted in a lower proportion of clumps and in smaller clumps.

AB - Mycelial fragmentation in submerged cultures of the cephalosporin C (CPC) producing fungus Acremonium chrysogenum was characterized by image analysis. In both fed-batch and chemostat cultures, the proportion of mycelial clumps seemed to be the most sensitive morphological indicator of fragmentation. In a fed-batch fermentation culture, this declined from roughly 60% at inoculation to less than 10% after 43 h. Subsequent additions of glucose resulted in a sharp increase back to near the initial value, an increase that reversed itself a few hours after glucose exhaustion. Meanwhile CPC production continued to decline steadily. On the other hand, the addition of soybean oil enhanced CPC production, but had no significant effect on the morphology. Although it may sometimes appear that morphology and productivity are related in batch or fed-batch cultures, this study suggests that this is because both respond simultaneously to more fundamental physiological changes, dependent on the availability of carbon. In circumstances, such as supplementary carbon source addition, the relationship is lost. Chemostat cultures supported this belief, as CPC-production rates were hardly affected by the specific growth rate, but the morphology showed significant differences, i.e., lower dilution rates resulted in a lower proportion of clumps and in smaller clumps.

KW - Acremonium chrysogenum

KW - Cephalosporin C

KW - Fragmentation

KW - Image analysis

KW - Morphology

UR - http://www.scopus.com/inward/record.url?scp=0034755890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034755890&partnerID=8YFLogxK

U2 - 10.1139/cjm-47-9-801

DO - 10.1139/cjm-47-9-801

M3 - Article

C2 - 11683461

AN - SCOPUS:0034755890

VL - 47

SP - 801

EP - 806

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

IS - 9

ER -