Analysis of the K+ current in human CD4+ T lymphocytes in hypercholesterolemic state

Sándor Somodi, András Balajthy, Orsolya Szilágyi, Zoltán Pethodoubleacute, M. Harangi, G. Paragh, G. Panyi, Péter Hajdu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Atherosclerosis involves immune mechanisms: T lymphocytes are found in atherosclerotic plaques, suggesting their activation during atherogenesis. The predominant voltage-gated potassium channel of T cells, Kv1.3 is a key regulator of the Ca2+-dependent activation pathway. In the present experiments we studied the proliferation capacity and functional changes of Kv1.3 channels in T cells from healthy and hypercholestaeremic patients.By means of CFSE-assay (carboxyfluorescein succinimidyl ester) we showed that spontaneous activation rate of lymphocytes in hypercholesterolemia was elevated and the antiCD3/antiCD28 co-stimulation was less effective as compared to the healthy group. Using whole-cell patch-clamping we obtained that the activation and deactivation kinetics of Kv1.3 channels were faster in hypercholesterolemic state but no change in other parameters of Kv1.3 were found (inactivation kinetics, steady-state activation, expression level). We suppose that incorporation of oxLDL species via its raft-rupturing effect can modify proliferative rate of T cells as well as the gating of Kv1.3 channels.

Original languageEnglish
Pages (from-to)20-26
Number of pages7
JournalCellular Immunology
Volume281
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

T-Lymphocytes
Atherosclerosis
Voltage-Gated Potassium Channels
Atherosclerotic Plaques
Lymphocyte Activation
Hypercholesterolemia
Constriction
Esters
oxidized low density lipoprotein
6-carboxyfluorescein
5-(6)-carboxyfluorescein diacetate succinimidyl ester

Keywords

  • Cholesterol
  • Kv1.3
  • Proliferation
  • T lymphocyte

ASJC Scopus subject areas

  • Immunology

Cite this

Analysis of the K+ current in human CD4+ T lymphocytes in hypercholesterolemic state. / Somodi, Sándor; Balajthy, András; Szilágyi, Orsolya; Pethodoubleacute, Zoltán; Harangi, M.; Paragh, G.; Panyi, G.; Hajdu, Péter.

In: Cellular Immunology, Vol. 281, No. 1, 01.2013, p. 20-26.

Research output: Contribution to journalArticle

Somodi, Sándor ; Balajthy, András ; Szilágyi, Orsolya ; Pethodoubleacute, Zoltán ; Harangi, M. ; Paragh, G. ; Panyi, G. ; Hajdu, Péter. / Analysis of the K+ current in human CD4+ T lymphocytes in hypercholesterolemic state. In: Cellular Immunology. 2013 ; Vol. 281, No. 1. pp. 20-26.
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