Analysis of the Function of Spire in Actin Assembly and Its Synergy with Formin and Profilin

Montserrat Bosch, Kim Ho Diep Le, B. Bugyi, John J. Correia, Louis Renault, Marie France Carlier

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The Spire protein, together with the formin Cappuccino and profilin, plays an important role in actin-based processes that establish oocyte polarity. Spire contains a cluster of four actin-binding WH2 domains. It has been shown to nucleate actin filaments and was proposed to remain bound to their pointed ends. Here we show that the multifunctional character of the WH2 domains allows Spire to sequester four G-actin subunits binding cooperatively in a tight SA4 complex and to nucleate, sever, and cap filaments at their barbed ends. Binding of Spire to barbed ends does not affect the thermodynamics of actin assembly at barbed ends but blocks barbed end growth from profilin-actin. The resulting Spire-induced increase in profilin-actin concentration enhances processive filament assembly by formin. The synergy between Spire and formin is reconstituted in an in vitro motility assay, which provides a functional basis for the genetic interplay between Spire, formin, and profilin in oogenesis.

Original languageEnglish
Pages (from-to)555-568
Number of pages14
JournalMolecular Cell
Volume28
Issue number4
DOIs
Publication statusPublished - Nov 30 2007

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Profilins
Actins
Oogenesis
Actin Cytoskeleton
Thermodynamics
Oocytes
Growth

Keywords

  • CELLBIO

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Analysis of the Function of Spire in Actin Assembly and Its Synergy with Formin and Profilin. / Bosch, Montserrat; Le, Kim Ho Diep; Bugyi, B.; Correia, John J.; Renault, Louis; Carlier, Marie France.

In: Molecular Cell, Vol. 28, No. 4, 30.11.2007, p. 555-568.

Research output: Contribution to journalArticle

Bosch, Montserrat ; Le, Kim Ho Diep ; Bugyi, B. ; Correia, John J. ; Renault, Louis ; Carlier, Marie France. / Analysis of the Function of Spire in Actin Assembly and Its Synergy with Formin and Profilin. In: Molecular Cell. 2007 ; Vol. 28, No. 4. pp. 555-568.
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