Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment

Maria Cristina Medici, Fabio Tummolo, Vito Martella, Maria Cristina Arcangeletti, Flora de Conto, Carlo Chezzi, Enikő Fehér, Szilvia Marton, Adriana Calderaro, K. Bányai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Group C rotaviruses (RVC) are enteric pathogens of humans and animals. Whole-genome sequences are available only for few RVCs, leaving gaps in our knowledge about their genetic diversity. We determined the full-length genome sequence of two human RVCs (PR2593/ 2004 and PR713/2012), detected in Italy from hospital-based surveillance for rotavirus infection in 2004 and 2012. In the 11 RNA genomic segments, the two Italian RVCs segregated within separate intra-genotypic lineages showed variation ranging from 1.9% (VP6) to 15.9% (VP3) at the nucleotide level. Comprehensive analysis of human RVC sequences available in the databases allowed us to reveal the existence of at least two major genome configurations, defined as type I and type II. Human RVCs of type I were all associated with the M3 VP3 genotype, including the Italian strain PR2593/2004. Conversely, human RVCs of type II were all associated with the M2 VP3 genotype, including the Italian strain PR713/2012. Reassortant RVC strains between these major genome configurations were identified. Although only a few full-genome sequences of human RVCs, mostly of Asian origin, are available, the analysis of human RVC sequences retrieved from the databases indicates that at least two intra-genotypic RVC lineages circulate in European countries. Gathering more sequence data is necessary to develop a standardized genotype and intragenotypic lineage classification system useful for epidemiological investigations and avoiding confusion in the literature.

Original languageEnglish
Article number000497
Pages (from-to)1888-1898
Number of pages11
JournalJournal of General Virology
Volume97
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

Keywords

  • Full-Genome
  • Gastroenteritis
  • Genetic diversity
  • Group C Rotavirus
  • Phylogenetic analysis

ASJC Scopus subject areas

  • Medicine(all)
  • Virology

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    Medici, M. C., Tummolo, F., Martella, V., Arcangeletti, M. C., de Conto, F., Chezzi, C., Fehér, E., Marton, S., Calderaro, A., & Bányai, K. (2016). Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment. Journal of General Virology, 97(8), 1888-1898. [000497]. https://doi.org/10.1099/jgv.0.000497