Analysis of the equalization of inverted repeats and neurovirulence using a pseudorabies virus mutant strain altered at the U1/Ir junction

Z. Boldogkői, Attila Braun, István Medveczky, Róbert Glávits, Bence Gyúró, István Fodor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A recombinant pseudorabies (Aujeszky's disease) virus (PrV) designated as vE161ac was constructed by deleting a 3-kbp DNA segment spanning the junction of long and short components of the viral genome, and by replacing the deleted segment with a lacZ-expression cassette. The aim of constructing this mutant was (a) to determine whether the terminal repeat (Tr) can serve as a template for the regeneration of the internal repeat (Ir), and (b) whether this deletion causes a reduction in the neuroinvasiveness of the virus. To analyze the mechanism of equalization, revertant viruses were selected and structurally characterized from vE161ac infection of PK-15 cells, mice and pigs. Because all revertants acquired Ir sequences identical to that of the wild-type virus, the equalization process occurred using the Tr as a template to reconstitute the Ir. We also found that the recombinant virus vE161ac was virulent in both pigs and mice. The data are discussed in view of studies performed with similar PrV mutants by other authors.

Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalVirus Genes
Volume17
Issue number1
DOIs
Publication statusPublished - 1998

Fingerprint

Suid Herpesvirus 1
Viruses
Terminal Repeat Sequences
Swine
Pseudorabies
Viral Genome
Regeneration
DNA
Infection

Keywords

  • Equalization
  • Herpesvirus
  • Neurovirulence determinant
  • Pseudorabies (aujeszky's disease) virus

ASJC Scopus subject areas

  • Virology
  • Molecular Biology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Analysis of the equalization of inverted repeats and neurovirulence using a pseudorabies virus mutant strain altered at the U1/Ir junction. / Boldogkői, Z.; Braun, Attila; Medveczky, István; Glávits, Róbert; Gyúró, Bence; Fodor, István.

In: Virus Genes, Vol. 17, No. 1, 1998, p. 89-98.

Research output: Contribution to journalArticle

Boldogkői, Z. ; Braun, Attila ; Medveczky, István ; Glávits, Róbert ; Gyúró, Bence ; Fodor, István. / Analysis of the equalization of inverted repeats and neurovirulence using a pseudorabies virus mutant strain altered at the U1/Ir junction. In: Virus Genes. 1998 ; Vol. 17, No. 1. pp. 89-98.
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