Analysis of the complete genome sequence of a novel, pseudorabies virus strain isolated in Southeast Europe

Zsolt Csabai, Dóra Tombácz, Zoltán Deim, Michael Snyder, Z. Boldogkői

Research output: Contribution to journalArticle

Abstract

Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia. Results. In this study, a novel PRV strain was isolated and characterized. PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan. Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity. We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations. We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome. Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.

Original languageEnglish
Article number1806842
JournalCanadian Journal of Infectious Diseases and Medical Microbiology
Volume2019
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Suid Herpesvirus 1
Genome
Base Composition
Pseudorabies
Viruses
Serbia
Viral Genome
Point Mutation
China
Cell Culture Techniques
Economics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Analysis of the complete genome sequence of a novel, pseudorabies virus strain isolated in Southeast Europe. / Csabai, Zsolt; Tombácz, Dóra; Deim, Zoltán; Snyder, Michael; Boldogkői, Z.

In: Canadian Journal of Infectious Diseases and Medical Microbiology, Vol. 2019, 1806842, 01.01.2019.

Research output: Contribution to journalArticle

@article{0157ff0c4bf347c2ac2dbcecd3b83d03,
title = "Analysis of the complete genome sequence of a novel, pseudorabies virus strain isolated in Southeast Europe",
abstract = "Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia. Results. In this study, a novel PRV strain was isolated and characterized. PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan. Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity. We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations. We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome. Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.",
author = "Zsolt Csabai and D{\'o}ra Tomb{\'a}cz and Zolt{\'a}n Deim and Michael Snyder and Z. Boldogkői",
year = "2019",
month = "1",
day = "1",
doi = "10.1155/2019/1806842",
language = "English",
volume = "2019",
journal = "Canadian Journal of Infectious Diseases and Medical Microbiology",
issn = "1712-9532",
publisher = "Pulsus Group Inc.",

}

TY - JOUR

T1 - Analysis of the complete genome sequence of a novel, pseudorabies virus strain isolated in Southeast Europe

AU - Csabai, Zsolt

AU - Tombácz, Dóra

AU - Deim, Zoltán

AU - Snyder, Michael

AU - Boldogkői, Z.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia. Results. In this study, a novel PRV strain was isolated and characterized. PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan. Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity. We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations. We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome. Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.

AB - Background. Pseudorabies virus (PRV) is the causative agent of Aujeszky's disease giving rise to significant economic losses worldwide. Many countries have implemented national programs for the eradication of this virus. In this study, long-read sequencing was used to determine the nucleotide sequence of the genome of a novel PRV strain (PRV-MdBio) isolated in Serbia. Results. In this study, a novel PRV strain was isolated and characterized. PRV-MdBio was found to exhibit similar growth properties to those of another wild-type PRV, the strain Kaplan. Single-molecule real-time (SMRT) sequencing has revealed that the new strain differs significantly in base composition even from strain Kaplan, to which it otherwise exhibits the highest similarity. We compared the genetic composition of PRV-MdBio to strain Kaplan and the China reference strain Ea and obtained that radical base replacements were the most common point mutations preceding conservative and silent mutations. We also found that the adaptation of PRV to cell culture does not lead to any tendentious genetic alteration in the viral genome. Conclusion. PRV-MdBio is a wild-type virus, which differs in base composition from other PRV strains to a relatively large extent.

UR - http://www.scopus.com/inward/record.url?scp=85065765172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065765172&partnerID=8YFLogxK

U2 - 10.1155/2019/1806842

DO - 10.1155/2019/1806842

M3 - Article

AN - SCOPUS:85065765172

VL - 2019

JO - Canadian Journal of Infectious Diseases and Medical Microbiology

JF - Canadian Journal of Infectious Diseases and Medical Microbiology

SN - 1712-9532

M1 - 1806842

ER -