Analysis of the aromatic 1H NMR spectrum of chicken plasminogen kringle 4

A. M. Petros, M. Gyenes, L. Patthy, M. Lliná

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The intact kringle 4 domain of chicken plasminogen has been characterized by 1H NMR spectroscopy at 300 and 620 MHz in both the presence and absence of ε{lunate}-aminocaproic acid, an antiflbrinolytic drug. The study focuses on the aromatic resonances. Comparisons with spectra from human, porcine and bovine kringle 4 homologs indicates a strict conservancy of conformation, reflecting the underlying primary sequence homology, and leads to an unambiguous assignment of all the aromatic resonances, including those of Phe15 and His40 which are unique to the chicken domain. Conclusive evidence is found that the Tyr9 ring fluctuates between two states, one in which it flips fast and other in which it is severely hindered. Similarly, the Tyr64 side chain finds itself in a structurally constrained locus. The Trp62, Tyr64, and Trp72 aromatic resonances are most sensitive to ligand presence, supporting a previously reported model of the kringle 4 lysine-binding site. His40, Phe41, and Tyr74 are also perturbed by ligand indicating proximity to the site. In contrast, the Phe15 aromatic spectrum indicates a rather mobile phenyl ring which is insensitive to ligand presence, thus confirming the lesser importance of the corresponding segment within the first kringle loop in determining kringle structure and/or function.

Original languageEnglish
Pages (from-to)192-202
Number of pages11
JournalArchives of Biochemistry and Biophysics
Volume264
Issue number1
DOIs
Publication statusPublished - 1988

Fingerprint

Kringles
Plasminogen
Chickens
Nuclear magnetic resonance
Ligands
Aminocaproic Acid
Nuclear magnetic resonance spectroscopy
Lysine
Conformations
Binding Sites
Sequence Homology
Magnetic Resonance Spectroscopy
Pharmaceutical Preparations
Swine
Proton Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Analysis of the aromatic 1H NMR spectrum of chicken plasminogen kringle 4. / Petros, A. M.; Gyenes, M.; Patthy, L.; Lliná, M.

In: Archives of Biochemistry and Biophysics, Vol. 264, No. 1, 1988, p. 192-202.

Research output: Contribution to journalArticle

Petros, A. M. ; Gyenes, M. ; Patthy, L. ; Lliná, M. / Analysis of the aromatic 1H NMR spectrum of chicken plasminogen kringle 4. In: Archives of Biochemistry and Biophysics. 1988 ; Vol. 264, No. 1. pp. 192-202.
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