Analysis of the 77 K fluorescence emission and excitation spectra of isolated etioplast inner membranes

Béla Böddi, Margareta Ryberg, Christer Sundqvist

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Low-temperature fluorescence emission and excitation analyses revealed four discrete spectral forms of protochlorophyllide and/or protochlorophyll in the etioplast inner membranes isolated from leaves of wheat (Triticum aestivum cv. Kosack). The emission spectra were recorded with various excitation wavelengths between 400 and 500 nm. The excitation spectra were recorded for fluorescence emitted at various wavelengths between 620 and 740 nm. The spectra were analysed by a combination of various computer-aided methods including differential analysis, gaussian resolution and topological plots. The four pigment forms can be characterized by their different excitation (Ex) and emission (Em) bands. Adding the satellite bands of the Qy(0-0) transitions, the emission vibrational (Emv) bands, with correlated amplitudes gave the following delineation: Ex436-Em633-Emv690, Ex443-Em645-Emv711, Ex451-Em657-Emv727, and Ex463-Em671-Emv740. The differences in the spectral properties of the four pigment forms are discussed in relation to possible differences in their structural arrangement, association to NADPH-protochlorophyllide oxidoreductase and localization in prolamellar bodies and/or prothylakoids.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume21
Issue number2-3
DOIs
Publication statusPublished - 1993

Fingerprint

etioplasts
protochlorophyllide reductase
Chloroplasts
Pigments
Triticum
emission spectra
Protochlorophyllide
Fluorescence
fluorescence
membranes
Membranes
protochlorophyllides
Wavelength
pigments
excitation
wavelengths
protochlorophyll
Satellites
Temperature
wheat

Keywords

  • Etioplast
  • Fluorescence emission spectra
  • Fluorescence excitation spectra
  • Gaussian resolution
  • NADPH-protochlorophyllide oxidoreductase
  • Protochlorophyllide
  • Spectral components
  • Spectral forms

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Analysis of the 77 K fluorescence emission and excitation spectra of isolated etioplast inner membranes. / Böddi, Béla; Ryberg, Margareta; Sundqvist, Christer.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 21, No. 2-3, 1993, p. 125-133.

Research output: Contribution to journalArticle

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