Analysis of serum and seminal plasma after feeding ochratoxin A with breeding boars

L. Solti, Tamás Pécsi, I. Barna-Vetró, Ferenc Szász, Krisztina Biró, Erzsébet Szabó

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The aim of the experiment was to investigate whether or not ochratoxin A (OA) can be detected in seminal plasma after feeding the toxin in five and 10 times of the human tolerable daily intake with breeding boars and how toxin profiles of serum and seminal plasma correspond to each other. In addition to that, the effect of the toxin challenge on motility and longevity of boar semen was also evaluated. OA from samples was analyzed by microplate ELISA. Percentage of progressive motility of spermatozoa was determined initially and after 24, 48, 96, 120 and 144 h of storage. OA appeared in serum and seminal plasma shortly after toxin application had started. Significant reduction of initial motility and impaired longevity was observed after toxin withdrawal. These findings suggest that OA might have the potential to affect sperm production and semen quality of boars, but further research is required to elucidate whether OA exerts direct effect on germinal epithelium or disturbs sperm cell maturation only.

Original languageEnglish
Pages (from-to)123-132
Number of pages10
JournalAnimal Reproduction Science
Volume56
Issue number2
DOIs
Publication statusPublished - Jun 28 1999

Fingerprint

seminal plasma
ochratoxin A
Semen
boars
Breeding
toxins
breeding
Serum
Spermatozoa
semen
spermatozoa
Sperm Maturation
No-Observed-Adverse-Effect Level
acceptable daily intake
Semen Analysis
sperm motility
epithelium
Epithelium
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay

Keywords

  • ELISA
  • Longevity
  • Motility
  • Ochratoxin A
  • Seminal plasma
  • Serum
  • Swine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Genetics

Cite this

Analysis of serum and seminal plasma after feeding ochratoxin A with breeding boars. / Solti, L.; Pécsi, Tamás; Barna-Vetró, I.; Szász, Ferenc; Biró, Krisztina; Szabó, Erzsébet.

In: Animal Reproduction Science, Vol. 56, No. 2, 28.06.1999, p. 123-132.

Research output: Contribution to journalArticle

Solti, L. ; Pécsi, Tamás ; Barna-Vetró, I. ; Szász, Ferenc ; Biró, Krisztina ; Szabó, Erzsébet. / Analysis of serum and seminal plasma after feeding ochratoxin A with breeding boars. In: Animal Reproduction Science. 1999 ; Vol. 56, No. 2. pp. 123-132.
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