Analysis of pralidoxime in serum, brain and CSF of rats

Huba Kalász, Éva Szöko, Tamás Tábi, Georg A. Petroianu, Dietrich E. Lorke, Abdulrab Omar, Salem Alafifi, Almerri Jasem, Kornélia Tekes

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 - 200 μg/mL, 0.3 - 7 μg/mL and 0.1 - 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoxime-type antidotes clinically used in organophosphate poisoning.

Original languageEnglish
Pages (from-to)237-241
Number of pages5
JournalMedicinal Chemistry
Volume5
Issue number3
DOIs
Publication statusPublished - Sep 18 2009

Keywords

  • Brain
  • CZE
  • Cerebrospinal fluid (CSF)
  • Pralidoxime
  • Serum

ASJC Scopus subject areas

  • Drug Discovery

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