Analysis of papillomavirus consensus L1 gene in a closed colony of baboons (Papio anubis)

Donald E. Wonderly, Philip J. Chan, S. Cseh, John D. Jacobson, Leonard Bailey

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: The purpose of this study was to analyze cervical specimens and semen from a closed colony of baboons for the presence of the papillomavirus consensus L1 gene. STUDY DESIGN: Cervical swabs were collected from lightly anesthetized female baboons. Semen was collected from a male baboon by standard electroejaculation techniques. Deoxyribonucleic acid was extracted from the cells by two different methods and analyzed by polymerase chain reaction targeting the L1 consensus gene common for >25 genital papillomaviruses. RESULTS: Analyses of the polymerase chain reaction- amplified products did not reveal bands for the papillomavirus in either the cervical specimens or the semen. CONCLUSIONS: The hypothesis of a linkage between primates with papillomavirus as a common factor is not supported by the results of this study. This information is also important in assisting clinicians in setting up specific pathogen-free colonies of baboons.

Original languageEnglish
Pages (from-to)1016-1017
Number of pages2
JournalAmerican Journal of Obstetrics and Gynecology
Volume182
Issue number5
DOIs
Publication statusPublished - 2000

Fingerprint

Papio anubis
Papio
Semen
Genes
Specific Pathogen-Free Organisms
Polymerase Chain Reaction
Primates
DNA

Keywords

  • Consensus gene
  • Nonhuman primates
  • Papillomavirus
  • Papio anubis
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynaecology

Cite this

Analysis of papillomavirus consensus L1 gene in a closed colony of baboons (Papio anubis). / Wonderly, Donald E.; Chan, Philip J.; Cseh, S.; Jacobson, John D.; Bailey, Leonard.

In: American Journal of Obstetrics and Gynecology, Vol. 182, No. 5, 2000, p. 1016-1017.

Research output: Contribution to journalArticle

Wonderly, Donald E. ; Chan, Philip J. ; Cseh, S. ; Jacobson, John D. ; Bailey, Leonard. / Analysis of papillomavirus consensus L1 gene in a closed colony of baboons (Papio anubis). In: American Journal of Obstetrics and Gynecology. 2000 ; Vol. 182, No. 5. pp. 1016-1017.
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