Analysis of papillomavirus consensus L1 gene in a closed colony of baboons (Papio anubis)

Donald E. Wonderly, Philip J. Chan, Sandor Cseh, John D. Jacobson, Leonard Bailey

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Abstract

OBJECTIVE: The purpose of this study was to analyze cervical specimens and semen from a closed colony of baboons for the presence of the papillomavirus consensus L1 gene. STUDY DESIGN: Cervical swabs were collected from lightly anesthetized female baboons. Semen was collected from a male baboon by standard electroejaculation techniques. Deoxyribonucleic acid was extracted from the cells by two different methods and analyzed by polymerase chain reaction targeting the L1 consensus gene common for >25 genital papillomaviruses. RESULTS: Analyses of the polymerase chain reaction- amplified products did not reveal bands for the papillomavirus in either the cervical specimens or the semen. CONCLUSIONS: The hypothesis of a linkage between primates with papillomavirus as a common factor is not supported by the results of this study. This information is also important in assisting clinicians in setting up specific pathogen-free colonies of baboons.

Original languageEnglish
Pages (from-to)1016-1017
Number of pages2
JournalAmerican journal of obstetrics and gynecology
Volume182
Issue number5
DOIs
Publication statusPublished - Jan 1 2000

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Keywords

  • Consensus gene
  • Nonhuman primates
  • Papillomavirus
  • Papio anubis
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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