Analysis of oxylipins in human plasma: Comparison of ultrahigh-performance liquid chromatography and ultrahigh-performance supercritical fluid chromatography coupled to mass spectrometry

R. Berkecz, Miroslav Lísa, Michal Holčapek

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The potential of ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC/MS) and ultrahigh-performance supercritical fluid chromatography (UHPSFC) coupled to negative-ion electrospray ionization mass spectrometry (ESI–MS) for the analysis of 46 oxylipins and 2 fatty acid standards is compared in terms of their chromatographic resolution with the emphasis on distinguishing isobaric interferences and the method sensitivity. UHPLC provides the baseline separation of 24 isobaric oxylipins within 13 min, while UHPSFC enables the separation of only 20 isobaric oxylipins within 8 min. Moreover, the UHPLC/ESI–MS method provides an average improvement of sensitivity by 3.5-fold. A similar trend is observed in the analysis of human plasma samples, but lower ion suppression effects caused by lysophospholipids (LPL) are observed in case of UHPSFC/ESI–MS due to better separation of LPL. Both methods are fully applicable for the analysis of oxylipins, but UHPLC/ESI–MS method is preferred due to better separation and higher sensitivity, which results in the identification of 31 oxylipins in human plasma based on available standards and additional tentative 20 identifications based on accurate m/z values and the fragmentation behavior known from the literature.

Original languageEnglish
Pages (from-to)107-121
Number of pages15
JournalJournal of Chromatography A
Volume1511
DOIs
Publication statusPublished - Aug 18 2017

Fingerprint

Oxylipins
Supercritical Fluid Chromatography
Plasma (human)
Liquid chromatography
Electrospray ionization
Liquid Chromatography
Electrospray Ionization Mass Spectrometry
Mass spectrometry
Mass Spectrometry
Lysophospholipids
Ions
Spectrometry
Spectrum Analysis
Negative ions
Fatty Acids
Liquids

Keywords

  • Eicosanoid
  • ESI
  • Oxylipin
  • Plasma
  • Prostaglandin
  • SPE
  • UHPLC/MS
  • UHPSFC/MS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

@article{3ed32623867b470f90d425aa44da309a,
title = "Analysis of oxylipins in human plasma: Comparison of ultrahigh-performance liquid chromatography and ultrahigh-performance supercritical fluid chromatography coupled to mass spectrometry",
abstract = "The potential of ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC/MS) and ultrahigh-performance supercritical fluid chromatography (UHPSFC) coupled to negative-ion electrospray ionization mass spectrometry (ESI–MS) for the analysis of 46 oxylipins and 2 fatty acid standards is compared in terms of their chromatographic resolution with the emphasis on distinguishing isobaric interferences and the method sensitivity. UHPLC provides the baseline separation of 24 isobaric oxylipins within 13 min, while UHPSFC enables the separation of only 20 isobaric oxylipins within 8 min. Moreover, the UHPLC/ESI–MS method provides an average improvement of sensitivity by 3.5-fold. A similar trend is observed in the analysis of human plasma samples, but lower ion suppression effects caused by lysophospholipids (LPL) are observed in case of UHPSFC/ESI–MS due to better separation of LPL. Both methods are fully applicable for the analysis of oxylipins, but UHPLC/ESI–MS method is preferred due to better separation and higher sensitivity, which results in the identification of 31 oxylipins in human plasma based on available standards and additional tentative 20 identifications based on accurate m/z values and the fragmentation behavior known from the literature.",
keywords = "Eicosanoid, ESI, Oxylipin, Plasma, Prostaglandin, SPE, UHPLC/MS, UHPSFC/MS",
author = "R. Berkecz and Miroslav L{\'i}sa and Michal Holčapek",
year = "2017",
month = "8",
day = "18",
doi = "10.1016/j.chroma.2017.06.070",
language = "English",
volume = "1511",
pages = "107--121",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",

}

TY - JOUR

T1 - Analysis of oxylipins in human plasma

T2 - Comparison of ultrahigh-performance liquid chromatography and ultrahigh-performance supercritical fluid chromatography coupled to mass spectrometry

AU - Berkecz, R.

AU - Lísa, Miroslav

AU - Holčapek, Michal

PY - 2017/8/18

Y1 - 2017/8/18

N2 - The potential of ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC/MS) and ultrahigh-performance supercritical fluid chromatography (UHPSFC) coupled to negative-ion electrospray ionization mass spectrometry (ESI–MS) for the analysis of 46 oxylipins and 2 fatty acid standards is compared in terms of their chromatographic resolution with the emphasis on distinguishing isobaric interferences and the method sensitivity. UHPLC provides the baseline separation of 24 isobaric oxylipins within 13 min, while UHPSFC enables the separation of only 20 isobaric oxylipins within 8 min. Moreover, the UHPLC/ESI–MS method provides an average improvement of sensitivity by 3.5-fold. A similar trend is observed in the analysis of human plasma samples, but lower ion suppression effects caused by lysophospholipids (LPL) are observed in case of UHPSFC/ESI–MS due to better separation of LPL. Both methods are fully applicable for the analysis of oxylipins, but UHPLC/ESI–MS method is preferred due to better separation and higher sensitivity, which results in the identification of 31 oxylipins in human plasma based on available standards and additional tentative 20 identifications based on accurate m/z values and the fragmentation behavior known from the literature.

AB - The potential of ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC/MS) and ultrahigh-performance supercritical fluid chromatography (UHPSFC) coupled to negative-ion electrospray ionization mass spectrometry (ESI–MS) for the analysis of 46 oxylipins and 2 fatty acid standards is compared in terms of their chromatographic resolution with the emphasis on distinguishing isobaric interferences and the method sensitivity. UHPLC provides the baseline separation of 24 isobaric oxylipins within 13 min, while UHPSFC enables the separation of only 20 isobaric oxylipins within 8 min. Moreover, the UHPLC/ESI–MS method provides an average improvement of sensitivity by 3.5-fold. A similar trend is observed in the analysis of human plasma samples, but lower ion suppression effects caused by lysophospholipids (LPL) are observed in case of UHPSFC/ESI–MS due to better separation of LPL. Both methods are fully applicable for the analysis of oxylipins, but UHPLC/ESI–MS method is preferred due to better separation and higher sensitivity, which results in the identification of 31 oxylipins in human plasma based on available standards and additional tentative 20 identifications based on accurate m/z values and the fragmentation behavior known from the literature.

KW - Eicosanoid

KW - ESI

KW - Oxylipin

KW - Plasma

KW - Prostaglandin

KW - SPE

KW - UHPLC/MS

KW - UHPSFC/MS

UR - http://www.scopus.com/inward/record.url?scp=85022049489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85022049489&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2017.06.070

DO - 10.1016/j.chroma.2017.06.070

M3 - Article

C2 - 28709649

AN - SCOPUS:85022049489

VL - 1511

SP - 107

EP - 121

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

ER -