Analysis of nonderivatized steroids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using C70 fullerene as matrix

Gergely Montsko, Alexandra Vaczy, Gabor Maasz, E. Mernyák, E. Frank, Csaba Bay, Zalan Kadar, R. Ohmacht, J. Wölfling, L. Márk

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Neutral steroid hormones are currently analyzed by gas or liquid chromatography/mass spectrometry based methods. Most of the steroid compounds, however, lack volatility and do not contain polar groups, which results in inadequate chromatographic behavior and low ionization efficiency. Derivatization of the steroids to form more volatile, thermostable, and charged products solves this difficulty, but the derivatization of compounds with unknown chemical moieties is not an easy task. In this study, a rapid, high-throughput, sensitive matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method is described using C70 fullerene as a matrix compound. The application of the method is demonstrated for five general sex steroids and for synthetic steroid compounds in both negative and positive ionization modes.

Original languageEnglish
Pages (from-to)869-874
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume395
Issue number3
DOIs
Publication statusPublished - 2009

Fingerprint

Ionization
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Steroids
Steroid hormones
Liquid chromatography
Volatilization
Gas chromatography
Gas Chromatography-Mass Spectrometry
Throughput
fullerene C70
Hormones

Keywords

  • Androgens
  • Endocrinology
  • Estrogens
  • Fullerene
  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
  • Steroid hormone

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Analysis of nonderivatized steroids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using C70 fullerene as matrix. / Montsko, Gergely; Vaczy, Alexandra; Maasz, Gabor; Mernyák, E.; Frank, E.; Bay, Csaba; Kadar, Zalan; Ohmacht, R.; Wölfling, J.; Márk, L.

In: Analytical and Bioanalytical Chemistry, Vol. 395, No. 3, 2009, p. 869-874.

Research output: Contribution to journalArticle

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AU - Mernyák, E.

AU - Frank, E.

AU - Bay, Csaba

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