Analysis of nitrate ion in nettle (Urtica dioica L.) by ion-pair chromatographic method on a C30 stationary phase

Zoltan Szabo, Katalin Böddi, L. Márk, Laszlo G. Szabo, R. Ohmacht

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Nitrate ion is a frequent pollutant not only in soil and natural water resources but in vegetables and foods as well. In our study we focused on nettle due to its increased ability to accumulate nitrate ions. A new, simple method for the separation and determination of nitrate ion based on reversed-phase ion-pair chromatography has been elaborated. A new four-step sample pretreatment method enables the precipitation of proteins and oxidative degradation of compounds that may disturb the identification of the nitrate ion: (1) extraction of the total nitrate content, (2) precipitation of proteins with acetonitrile, (3) oxidative degradation of the organic contaminants with H2O 2, (4) evaporation of the solvent and taking up of the residue in water. The chromatographic separations were carried out on a high-density C 30 stationary phase under isocratic conditions. The optimal mobile-phase composition was 10% (v/v) acetonitrile and 90% (v/v) 20 mmol L -1 phosphate buffer, containing 2 mmol of tetrabutylammonium hydroxide at pH 6.0. The method could also be used for the separation of IO 3-, SeO32-, BrO3 -, NO2-, Br-, SeO4 2-, and I- ions. The validated method is sensitive (the detection limit is 0.18 ng of nitrate ion). The method is linear in a high concentration range (0.031-30:66 μg mL-1). Recoveries varied between 98% and 103%. Reproducibility of the elaborated sample pretreatment method showed 1.54%. The method can be used for the determination of nitrate ion from different plants.

Original languageEnglish
Pages (from-to)4082-4086
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number12
DOIs
Publication statusPublished - Jun 14 2006

Fingerprint

Urtica dioica
Nitrates
nitrates
Ions
ions
methodology
ion pair chromatography
pretreatment
Ion chromatography
Degradation
degradation
hydroxides
Water Resources
Vegetables
Water resources
Phase composition
reproducibility
water resources
evaporation
Buffers

Keywords

  • C stationary phase
  • Nettle (Urtica dioica L.)
  • Nitrate ion
  • Reversed-phase ion-pair (RP-IP) chromatography

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Analysis of nitrate ion in nettle (Urtica dioica L.) by ion-pair chromatographic method on a C30 stationary phase. / Szabo, Zoltan; Böddi, Katalin; Márk, L.; Szabo, Laszlo G.; Ohmacht, R.

In: Journal of Agricultural and Food Chemistry, Vol. 54, No. 12, 14.06.2006, p. 4082-4086.

Research output: Contribution to journalArticle

@article{78395646d0244e56bac4305380346f51,
title = "Analysis of nitrate ion in nettle (Urtica dioica L.) by ion-pair chromatographic method on a C30 stationary phase",
abstract = "Nitrate ion is a frequent pollutant not only in soil and natural water resources but in vegetables and foods as well. In our study we focused on nettle due to its increased ability to accumulate nitrate ions. A new, simple method for the separation and determination of nitrate ion based on reversed-phase ion-pair chromatography has been elaborated. A new four-step sample pretreatment method enables the precipitation of proteins and oxidative degradation of compounds that may disturb the identification of the nitrate ion: (1) extraction of the total nitrate content, (2) precipitation of proteins with acetonitrile, (3) oxidative degradation of the organic contaminants with H2O 2, (4) evaporation of the solvent and taking up of the residue in water. The chromatographic separations were carried out on a high-density C 30 stationary phase under isocratic conditions. The optimal mobile-phase composition was 10{\%} (v/v) acetonitrile and 90{\%} (v/v) 20 mmol L -1 phosphate buffer, containing 2 mmol of tetrabutylammonium hydroxide at pH 6.0. The method could also be used for the separation of IO 3-, SeO32-, BrO3 -, NO2-, Br-, SeO4 2-, and I- ions. The validated method is sensitive (the detection limit is 0.18 ng of nitrate ion). The method is linear in a high concentration range (0.031-30:66 μg mL-1). Recoveries varied between 98{\%} and 103{\%}. Reproducibility of the elaborated sample pretreatment method showed 1.54{\%}. The method can be used for the determination of nitrate ion from different plants.",
keywords = "C stationary phase, Nettle (Urtica dioica L.), Nitrate ion, Reversed-phase ion-pair (RP-IP) chromatography",
author = "Zoltan Szabo and Katalin B{\"o}ddi and L. M{\'a}rk and Szabo, {Laszlo G.} and R. Ohmacht",
year = "2006",
month = "6",
day = "14",
doi = "10.1021/jf0524628",
language = "English",
volume = "54",
pages = "4082--4086",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "12",

}

TY - JOUR

T1 - Analysis of nitrate ion in nettle (Urtica dioica L.) by ion-pair chromatographic method on a C30 stationary phase

AU - Szabo, Zoltan

AU - Böddi, Katalin

AU - Márk, L.

AU - Szabo, Laszlo G.

AU - Ohmacht, R.

PY - 2006/6/14

Y1 - 2006/6/14

N2 - Nitrate ion is a frequent pollutant not only in soil and natural water resources but in vegetables and foods as well. In our study we focused on nettle due to its increased ability to accumulate nitrate ions. A new, simple method for the separation and determination of nitrate ion based on reversed-phase ion-pair chromatography has been elaborated. A new four-step sample pretreatment method enables the precipitation of proteins and oxidative degradation of compounds that may disturb the identification of the nitrate ion: (1) extraction of the total nitrate content, (2) precipitation of proteins with acetonitrile, (3) oxidative degradation of the organic contaminants with H2O 2, (4) evaporation of the solvent and taking up of the residue in water. The chromatographic separations were carried out on a high-density C 30 stationary phase under isocratic conditions. The optimal mobile-phase composition was 10% (v/v) acetonitrile and 90% (v/v) 20 mmol L -1 phosphate buffer, containing 2 mmol of tetrabutylammonium hydroxide at pH 6.0. The method could also be used for the separation of IO 3-, SeO32-, BrO3 -, NO2-, Br-, SeO4 2-, and I- ions. The validated method is sensitive (the detection limit is 0.18 ng of nitrate ion). The method is linear in a high concentration range (0.031-30:66 μg mL-1). Recoveries varied between 98% and 103%. Reproducibility of the elaborated sample pretreatment method showed 1.54%. The method can be used for the determination of nitrate ion from different plants.

AB - Nitrate ion is a frequent pollutant not only in soil and natural water resources but in vegetables and foods as well. In our study we focused on nettle due to its increased ability to accumulate nitrate ions. A new, simple method for the separation and determination of nitrate ion based on reversed-phase ion-pair chromatography has been elaborated. A new four-step sample pretreatment method enables the precipitation of proteins and oxidative degradation of compounds that may disturb the identification of the nitrate ion: (1) extraction of the total nitrate content, (2) precipitation of proteins with acetonitrile, (3) oxidative degradation of the organic contaminants with H2O 2, (4) evaporation of the solvent and taking up of the residue in water. The chromatographic separations were carried out on a high-density C 30 stationary phase under isocratic conditions. The optimal mobile-phase composition was 10% (v/v) acetonitrile and 90% (v/v) 20 mmol L -1 phosphate buffer, containing 2 mmol of tetrabutylammonium hydroxide at pH 6.0. The method could also be used for the separation of IO 3-, SeO32-, BrO3 -, NO2-, Br-, SeO4 2-, and I- ions. The validated method is sensitive (the detection limit is 0.18 ng of nitrate ion). The method is linear in a high concentration range (0.031-30:66 μg mL-1). Recoveries varied between 98% and 103%. Reproducibility of the elaborated sample pretreatment method showed 1.54%. The method can be used for the determination of nitrate ion from different plants.

KW - C stationary phase

KW - Nettle (Urtica dioica L.)

KW - Nitrate ion

KW - Reversed-phase ion-pair (RP-IP) chromatography

UR - http://www.scopus.com/inward/record.url?scp=33745755238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745755238&partnerID=8YFLogxK

U2 - 10.1021/jf0524628

DO - 10.1021/jf0524628

M3 - Article

C2 - 16756329

AN - SCOPUS:33745755238

VL - 54

SP - 4082

EP - 4086

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 12

ER -